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dc.contributor.authorKrey, Whitney B.  Concept link
dc.coverage.spatialCosta Rica Dome
dc.date.accessioned2008-09-04T16:20:45Z
dc.date.available2008-09-04T16:20:45Z
dc.date.issued2008-06
dc.identifier.urihttps://hdl.handle.net/1912/2394
dc.descriptionSubmitted in partial fulfillment of the requirements for the degree of Master of Science at the Massachusetts Institute of Technology and the Woods Hole Oceanographic Institution June 2008.en
dc.description.abstractAn increased understanding of heterotrophic bacterial strategies for acquiring nutrients and trace elements is critical for elucidating their impact on biogeochemical cycling in the ocean. It is estimated that iron is a limiting nutrient for phytoplankton growth in over 30% of the open ocean, but still little is known about bacterial strategies for iron acquisition. Siderophore (Fe ligand) production by bacteria may play a major role in influencing the bioavailability of iron in the ocean. Despite the importance of siderophores in the environment, only limited information from a select group of bacteria is available. On a cruise through the Costa Rica Dome (CRD) upwelling region in July 2005, a library of 867 isolates from five depth profiles inside and outside of the dome was obtained and screened for siderophore production using the Chrome Azurol-S (CAS) assay. Phylogenetic affiliation of 134 isolates was determined by sequencing the 16s rDNA gene, and determined that gamma proteobacteria such as Alteromonas, Pseudoalteromonas, Halomonas, and Marinobacter dominated the collection, while alpha-proteobacteria such as Roseobacter were also represented. The isolates obtained from stations in the CRD showed greater siderophore-producing capabilities between 55m and 100m while strains isolated from outside the CRD had shallower peak (~8-35m) production. Functional group determination showed that hydroxamate production dominated from 50-150m, while hydroxamate and catechol production is roughly equal in shallower waters. By characterizing the siderophores produced by these isolates and determining the genetic make-up of the population, these findings further our understanding of how heterotrophic microbes affect biogeochemical processes and the competitive nature of nutrient acquisition.en
dc.description.sponsorshipI am grateful to my funding sources, National Science Foundation grants BO OCE- 0352241 "The Effect of Iron Bioavailability on Synechococcus diversity from a HNLC region to the Costa Rica upwelling dome" and CO-OCE-0452883 "Interactions of Cobalt and Iron with in situ Cyanobacterial Physiology in the South Atlantic and the Benguela Upwelling Region", the National Science Foundation Graduate Research Fellowship, and the Academic Programs office.en
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen
dc.publisherMassachusetts Institute of Technology and Woods Hole Oceanographic Institutionen
dc.relation.ispartofseriesWHOI Thesesen
dc.subjectSiderophoresen_US
dc.subjectHeterotrophic bacteriaen_US
dc.subjectKnorr (Ship : 1970-) Cruise KN182-50en_US
dc.titleSiderophore production by heterotrophic bacterial isolates from the Costa Rica Upwelling Domeen
dc.typeThesisen
dc.identifier.doi10.1575/1912/2394


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