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dc.contributor.authorEdzuka, Tomoya  Concept link
dc.contributor.authorGoshima, Gohta  Concept link
dc.identifier.citationEdzuka, T., & Goshima, G. (2019). Drosophila kinesin-8 stabilizes the kinetochore-microtubule interaction. Journal of Cell Biology, 218(2), 474-488en_US
dc.description© The Authors, 2019. This article is distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 4.0 International License. The definitive version was published in Journal of Cell Biology 218(2), (2019): 474-488, doi: 10.1083/jcb.201807077.en_US
dc.description.abstractKinesin-8 is required for proper chromosome alignment in a variety of animal and yeast cell types. However, it is unclear how this motor protein family controls chromosome alignment, as multiple biochemical activities, including inconsistent ones between studies, have been identified. Here, we find that Drosophila kinesin-8 (Klp67A) possesses both microtubule (MT) plus end–stabilizing and –destabilizing activity, in addition to kinesin-8's commonly observed MT plus end–directed motility and tubulin-binding activity in vitro. We further show that Klp67A is required for stable kinetochore–MT attachment during prometaphase in S2 cells. In the absence of Klp67A, abnormally long MTs interact in an “end-on” fashion with kinetochores at normal frequency. However, the interaction is unstable, and MTs frequently become detached. This phenotype is rescued by ectopic expression of the MT plus end–stabilizing factor CLASP, but not by artificial shortening of MTs. We show that human kinesin-8 (KIF18A) is also important to ensure proper MT attachment. Overall, these results suggest that the MT-stabilizing activity of kinesin-8 is critical for stable kinetochore–MT attachment.en_US
dc.description.sponsorshipWe thank Kosuke Ariga for helping data analysis, Tomoko Nishiyama for technical support, Tomomi Kiyomitsu for valuable comments on the manuscript, and Elsa Tungadi for proofreading. This work was funded by Japan Society for the Promotion of Science (JSPS) KAK ENHI (15KT0077 and 17H01431) and Laura and Arthur Colwin Endowed Summer Research Fellowship Fund (2015) of the Marine Biological Laboratory to G. Goshima. T. Edzuka is a recipient of a JSPS pre-doctoral fellowship (16J02807).en_US
dc.publisherRockefeller University Pressen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.titleDrosophila kinesin-8 stabilizes the kinetochore–microtubule interactionen_US

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