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dc.contributor.authorHanslik, Kendra  Concept link
dc.contributor.authorAllen, Scott R.  Concept link
dc.contributor.authorHarkenrider, Tessa L.  Concept link
dc.contributor.authorFogerson, Stephanie M.  Concept link
dc.contributor.authorGuadarrama, Eduardo  Concept link
dc.contributor.authorMorgan, Jennifer R.  Concept link
dc.date.accessioned2019-02-20T15:09:16Z
dc.date.available2019-02-20T15:09:16Z
dc.date.issued2019-01-30
dc.identifier.citationHanslik, K. L., Allen, S. R., Harkenrider, T. L., Fogerson, S. M., Guadarrama, E., & Morgan, J. R. (2019). Regenerative capacity in the lamprey spinal cord is not altered after a repeated transection. Plos One, 14(1), e0204193en_US
dc.identifier.urihttps://hdl.handle.net/1912/23683
dc.description© The Author(s), 2019. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLoS One 14(1), (2019):e0204193, doi: 10.1371/journal.pone.0204193.en_US
dc.description.abstractThe resilience of regeneration in vertebrates is not very well understood. Yet understanding if tissues can regenerate after repeated insults, and identifying limitations, is important for elucidating the underlying mechanisms of tissue plasticity. This is particularly challenging in tissues, such as the nervous system, which possess a large number of terminally differentiated cells and often exhibit limited regeneration in the first place. However, unlike mammals, which exhibit very limited regeneration of spinal cord tissues, many non-mammalian vertebrates, including lampreys, bony fishes, amphibians, and reptiles, regenerate their spinal cords and functionally recover even after a complete spinal cord transection. It is well established that lampreys undergo full functional recovery of swimming behaviors after a single spinal cord transection, which is accompanied by tissue repair at the lesion site, as well as axon and synapse regeneration. Here we begin to explore the resilience of spinal cord regeneration in lampreys after a second spinal transection (re-transection). We report that by all functional and anatomical measures tested, lampreys regenerate after spinal re-transection just as robustly as after single transections. Recovery of swimming, synapse and cytoskeletal distributions, axon regeneration, and neuronal survival were nearly identical after spinal transection or re-transection. Only minor differences in tissue repair at the lesion site were observed in re-transected spinal cords. Thus, regenerative potential in the lamprey spinal cord is largely unaffected by spinal re-transection, indicating a greater persistent regenerative potential than exists in some other highly regenerative models. These findings establish a new path for uncovering pro-regenerative targets that could be deployed in non-regenerative conditions.en_US
dc.description.sponsorshipThe authors would like to thank Dr. Cristina Roman-Vendrell and Louie Kerr, Director of the Central Microscopy Facility at the MBL, for technical support. We also thank Dr. Juan Diaz-Quiroz for helpful comments on the manuscript. EG was supported in part by an NSF REU Award (#1659604: Biological Discovery in Woods Hole at the Marine Biological Laboratory).en_US
dc.publisherPublic Library of Scienceen_US
dc.relation.urihttps://doi.org/10.1371/journal.pone.0204193
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleRegenerative capacity in the lamprey spinal cord is not altered after a repeated transectionen_US
dc.typeArticleen_US
dc.identifier.doi10.1371/journal.pone.0204193


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International