Improvements in simultaneous sodium and calcium imaging
MetadataShow full item record
High speed imaging of ion concentration changes in neurons is an important and growing tool for neuroscientists. We previously developed a system for simultaneously measuring sodium and calcium changes in small compartments in neurons (Miyazaki and Ross, 2015). We used this technique to analyze the dynamics of these ions in individual pyramidal neuron dendritic spines (Miyazaki and Ross, 2017). This system is based on high speed multiplexing of light emitting diodes (LEDs) and classic organic indicators. To improve this system we made additional changes, primarily incorporating lasers in addition to the LEDs, more sophisticated imaging protocols, and the use of newer sodium and calcium indicators. This new system generates signals with higher signal to noise ratio (S/N), less background fluorescence, and less photodynamic damage. In addition, by using longer wavelength indicators instead of indicators sensitive in the UV range, it allows for the incorporation of focal uncaging along with simultaneous imaging, which should extend the range of experiments.
© The Author(s), 2019. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Frontiers in Cellular Neuroscience 12 (2019): 514. doi: 10.3389/fncel.2018.00514.
Suggested CitationImprovements in simultaneous sodium and calcium imaging
The following license files are associated with this item:
Showing items related by title, author, creator and subject.
Miyazaki, Kenichi; Ross, William N. (Society for Neuroscience, 2015-10-14)Dynamic calcium imaging is a major technique of neuroscientists. It can reveal information about the location of various calcium channels and calcium permeable receptors, the time course, magnitude, and location of ...
Prolonged calcium influx after termination of light-induced calcium release in invertebrate photoreceptors Gomez, Maria del Pilar; Nasi, Enrico (Rockefeller University Press, 2009-08-31)In microvillar photoreceptors, light stimulates the phospholipase C cascade and triggers an elevation of cytosolic Ca2+ that is essential for the regulation of both visual excitation and sensory adaptation. In some organisms, ...
Barzilai, Maya Gur; Reitzel, Adam M.; Kraus, Johanna E. M.; Gordon, Dalia; Technau, Ulrich; Gurevitz, Michael; Moran, Yehu (Cell Press, 2012-08-30)Ion selectivity of metazoan voltage-gated Na+ channels is critical for neuronal signaling and has long been attributed to a ring of four conserved amino acids that constitute the ion selectivity filter (SF) at the channel ...