Cloning a new cytochrome P450 isoform (CYP356A1) from oyster Crassostrea gigas
de Toledo-Silva, Guilherme
Siebert, Marília N.
Medeiros, Igor D.
Sincero, Thaís C. M.
Moraes, Milton O.
Goldstone, Jared V.
Bainy, Afonso C. D.
MetadataShow full item record
KeywordCytochrome P450; Pacific oyster; Crassostrea gigas; Biotransformation enzymes; CYP356A1; CYP17
We have cloned the full-length cDNA of the first member of a new cytochrome P450 (CYP) family from the Pacific oyster Crassostrea gigas. This new CYP gene was obtained based on an initial 331 bp fragment previously identified among the list of the differentially expressed genes in oysters exposed to untreated domestic sewage. The full-length CYP has an open reading frame of 1500 bp and based on its deduced aminoacid sequence was classified as a member of a new subfamily, CYP356A1. A phylogenetic analysis showed that CYP356A1 is closely related to members of the CYP17 and CYP1 subfamilies. Semiquantitative RT-PCR was performed to analyze the CYP356A1 expression in different tissues of the oyster (digestive gland, gill, mantle and adductor muscle). Results showed slightly higher CYP356A1 expression in digestive gland and mantle, than the other tissues, indicating a possible role of the CYP356A1 in the xenobiotic biotransformation and/or steroid metabolism.
Author Posting. © Elsevier B.V., 2008. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Marine Environmental Research 66 (2008): 15-18, doi:10.1016/j.marenvres.2008.02.010.
Suggested CitationPreprint: de Toledo-Silva, Guilherme, Siebert, Marília N., Medeiros, Igor D., Sincero, Thaís C. M., Moraes, Milton O., Goldstone, Jared V., Bainy, Afonso C. D., "Cloning a new cytochrome P450 isoform (CYP356A1) from oyster Crassostrea gigas", 2008-02, https://doi.org/10.1016/j.marenvres.2008.02.010, https://hdl.handle.net/1912/2332
Showing items related by title, author, creator and subject.
Light stimulates swimming behavior of larval eastern oysters Crassostrea virginica in turbulent flow Wheeler, Jeanette D.; Luo, Elaine; Helfrich, Karl R.; Anderson, Erik J.; Starczak, Victoria R.; Mullineaux, Lauren S. (2017-05)Planktonic larvae of the eastern oyster Crassostrea virginica are able to regulate their vertical position in the water, but the environmental cues responsible for this regulation, particularly in turbulent settings, remain ...
Upward swimming of competent oyster larvae Crassostrea virginica persists in highly turbulent flow as detected by PIV flow subtraction Wheeler, Jeanette D.; Helfrich, Karl R.; Anderson, Erik J.; McGann, B.; Staats, P.; Wargula, Anna E.; Wilt, K.; Mullineaux, Lauren S. (Inter-Research, 2013-08-15)Investigating settlement responses in the transitory period between planktonic and benthic stages of invertebrates helps shape our understanding of larval dispersal and supply, as well as early adult survival. Turbulence ...
Development and validation of a real-time quantitative PCR assay for the detection and quantification of Perkinsus marinus in the Eastern oyster, Crassostrea virginica DeFaveri, Jacquelin; Smolowitz, Roxanna M.; Roberts, Steven B. (National Shellfisheries Association, 2009-08)Perkinus marinus causes a devastating disease, known as Dermo, in the Eastern oyster Crassostrea virginica. Routine detection of the disease is traditionally accomplished by the use of the Ray/Makin assay, using Fluid ...