Cloning a new cytochrome P450 isoform (CYP356A1) from oyster Crassostrea gigas
de Toledo-Silva, Guilherme
Siebert, Marília N.
Medeiros, Igor D.
Sincero, Thaís C. M.
Moraes, Milton O.
Goldstone, Jared V.
Bainy, Afonso C. D.
MetadataShow full item record
KeywordCytochrome P450; Pacific oyster; Crassostrea gigas; Biotransformation enzymes; CYP356A1; CYP17
We have cloned the full-length cDNA of the first member of a new cytochrome P450 (CYP) family from the Pacific oyster Crassostrea gigas. This new CYP gene was obtained based on an initial 331 bp fragment previously identified among the list of the differentially expressed genes in oysters exposed to untreated domestic sewage. The full-length CYP has an open reading frame of 1500 bp and based on its deduced aminoacid sequence was classified as a member of a new subfamily, CYP356A1. A phylogenetic analysis showed that CYP356A1 is closely related to members of the CYP17 and CYP1 subfamilies. Semiquantitative RT-PCR was performed to analyze the CYP356A1 expression in different tissues of the oyster (digestive gland, gill, mantle and adductor muscle). Results showed slightly higher CYP356A1 expression in digestive gland and mantle, than the other tissues, indicating a possible role of the CYP356A1 in the xenobiotic biotransformation and/or steroid metabolism.
Author Posting. © Elsevier B.V., 2008. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Marine Environmental Research 66 (2008): 15-18, doi:10.1016/j.marenvres.2008.02.010.
Suggested CitationPreprint: de Toledo-Silva, Guilherme, Siebert, Marília N., Medeiros, Igor D., Sincero, Thaís C. M., Moraes, Milton O., Goldstone, Jared V., Bainy, Afonso C. D., "Cloning a new cytochrome P450 isoform (CYP356A1) from oyster Crassostrea gigas", 2008-02, https://doi.org/10.1016/j.marenvres.2008.02.010, https://hdl.handle.net/1912/2332
Showing items related by title, author, creator and subject.
Modelling interactions of acid–base balance and respiratory status in the toxicity of metal mixtures in the American oyster Crassostrea virginica Macey, Brett M.; Jenny, Matthew J.; Williams, Heidi R.; Thibodeaux, Lindy K.; Beal, Marion; Almeida, Jonas S.; Cunningham, Charles; Mancia, Annalaura; Warr, Gregory W.; Burge, Erin J.; Holland, A. Fredrick; Gross, Paul S.; Hikima, Sonomi; Burnett, Karen G.; Burnett, Louis; Chapman, Robert W. (2009-11-12)Heavy metals, such as copper, zinc and cadmium, represent some of the most common and serious pollutants in coastal estuaries. In the present study, we used a combination of linear and artificial neural network (ANN) ...
Development and validation of a real-time quantitative PCR assay for the detection and quantification of Perkinsus marinus in the Eastern oyster, Crassostrea virginica DeFaveri, Jacquelin; Smolowitz, Roxanna M.; Roberts, Steven B. (National Shellfisheries Association, 2009-08)Perkinus marinus causes a devastating disease, known as Dermo, in the Eastern oyster Crassostrea virginica. Routine detection of the disease is traditionally accomplished by the use of the Ray/Makin assay, using Fluid ...
Uptake of heavy metals, organic trace contaminants and viruses by the Japanese oyster, Crassostrea gigas, grown in a waste recycling aquaculture system : final report Mann, Roger; Vaughn, James M.; Landry, Edward F.; Taylor, Rodman E. (Woods Hole Oceanographic Institution, 1979-05)A study of 24 weeks duration was carried out in which oysters (Crassostrea gigas) were grown in four regimes. These were: (i) on phytoplankton cultured in a mixture of secondary treated sewage effluent and seawater for ...