• Login
    About WHOAS
    View Item 
    •   WHOAS Home
    • Woods Hole Oceanographic Institution
    • Biology
    • View Item
    •   WHOAS Home
    • Woods Hole Oceanographic Institution
    • Biology
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of WHOASCommunities & CollectionsBy Issue DateAuthorsTitlesKeywordsThis CollectionBy Issue DateAuthorsTitlesKeywords

    My Account

    LoginRegister

    Statistics

    View Usage Statistics

    Development of the morpholino gene knockdown technique in Fundulus heteroclitus : a tool for studying molecular mechanisms in an established environmental model

    Thumbnail
    View/Open
    Fundulus morpholino Manuscript FINAL Draft.pdf (691.5Kb)
    Date
    2008-02-13
    Author
    Matson, Cole W.  Concept link
    Clark, Bryan W.  Concept link
    Jenny, Matthew J.  Concept link
    Fleming, Carrie R.  Concept link
    Hahn, Mark E.  Concept link
    Di Giulio, Richard T.  Concept link
    Metadata
    Show full item record
    Citable URI
    https://hdl.handle.net/1912/2271
    As published
    https://doi.org/10.1016/j.aquatox.2008.02.010
    Keyword
     Fundulus heteroclitus; Antisense morpholino oligonucleotide; CYP1A; Atlantic killifish; Genetic toxicology; Development; Teratogenesis 
    Abstract
    A significant challenge in environmental toxicology is that many genetic and genomic tools available in laboratory models are not developed for commonly used environmental models. The Atlantic killifish (Fundulus heteroclitus) is one of the most studied teleost environmental models, yet few genetic or genomic tools have been developed for use in this species. The advancement of genetic and evolutionary toxicology will require that many of the tools developed in laboratory models be transferred into species more applicable to environmental toxicology. Antisense morpholino oligonucleotide (MO) gene knockdown technology has been widely utilized to study development in zebrafish and has been proven to be a powerful tool in toxicological investigations through direct manipulation of molecular pathways. To expand the utility of killifish as an environmental model, MO gene knockdown technology was adapted for use in Fundulus. Morpholino microinjection methods were altered to overcome the significant differences between these two species. Morpholino efficacy and functional duration were evaluated with molecular and phenotypic methods. A cytochrome P450-1A (CYP1A) MO was used to confirm effectiveness of the methodology. For CYP1A MO-injected embryos, a 70% reduction in CYP1A activity, a 86% reduction in total CYP1A protein, a significant increase in β-naphthoflavone-induced teratogenicity, and estimates of functional duration (50% reduction in activity 10 dpf, and 86% reduction in total protein 12 dpf) conclusively demonstrated that MO technologies can be used effectively in killifish and will likely be just as informative as they have been in zebrafish.
    Description
    Author Posting. © Elsevier B.V., 2008. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Aquatic Toxicology 87 (2008): 289-295, doi:10.1016/j.aquatox.2008.02.010.
    Collections
    • Biology
    Suggested Citation
    Preprint: Matson, Cole W., Clark, Bryan W., Jenny, Matthew J., Fleming, Carrie R., Hahn, Mark E., Di Giulio, Richard T., "Development of the morpholino gene knockdown technique in Fundulus heteroclitus : a tool for studying molecular mechanisms in an established environmental model", 2008-02-13, https://doi.org/10.1016/j.aquatox.2008.02.010, https://hdl.handle.net/1912/2271
     

    Related items

    Showing items related by title, author, creator and subject.

    • Thumbnail

      Development and characterization of polyclonal antibodies against the aryl hydrocarbon receptor protein family (AHR1, AHR2, and AHR repressor) of Atlantic killifish Fundulus heteroclitus 

      Merson, Rebeka R.; Franks, Diana G.; Karchner, Sibel I.; Hahn, Mark E. (2005-12-20)
      The aryl hydrocarbon receptor (AHR) and AHR repressor (AHRR) proteins regulate gene expression in response to some halogenated aromatic hydrocarbons and polycyclic aromatic hydrocarbons. The Atlantic killifish is a valuable ...
    • Thumbnail

      Can diversifying selection be distinguished from history in geographic clines? A population genomic study of killifish (Fundulus heteroclitus) 

      Strand, Allan E.; Williams, Larissa M.; Oleksiak, Marjorie F.; Sotka, Erik E. (Public Library of Science, 2012-09-26)
      A common geographical pattern of genetic variation is the one-dimensional cline. Clines may be maintained by diversifying selection across a geographical gradient but can also reflect historical processes such as allopatry ...
    • Thumbnail

      Turnover rates of nitrogen stable isotopes in the salt marsh mummichog, Fundulus heteroclitus, following a laboratory diet switch 

      Logan, John; Haas, Heather; Deegan, Linda A.; Gaines, Emily F. (2005-09-19)
      Nitrogen stable isotopes are frequently used in ecological studies to estimate trophic position and determine movement patterns. Knowledge of tissue-specific turnover and nitrogen discrimination for the study organisms is ...
    All Items in WHOAS are protected by original copyright, with all rights reserved, unless otherwise indicated. WHOAS also supports the use of the Creative Commons licenses for original content.
    A service of the MBLWHOI Library | About WHOAS
    Contact Us | Send Feedback | Privacy Policy