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dc.contributor.authorBucior, Iwona  Concept link
dc.contributor.authorScheuring, Simon  Concept link
dc.contributor.authorEngel, Andreas  Concept link
dc.contributor.authorBurger, Max M.  Concept link
dc.identifier.citationJournal of Cell Biology 165 (2004): 529-537en
dc.description© 2004 Bucior et al. This article is distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. The definitive version was published in Journal of Cell Biology 165 (2004): 529-537, doi:10.1083/jcb.200309005.en
dc.description.abstractThe adhesion force and specificity in the first experimental evidence for cell–cell recognition in the animal kingdom were assigned to marine sponge cell surface proteoglycans. However, the question whether the specificity resided in a protein or carbohydrate moiety could not yet be resolved. Here, the strength and species specificity of cell–cell recognition could be assigned to a direct carbohydrate–carbohydrate interaction. Atomic force microscopy measurements revealed equally strong adhesion forces between glycan molecules (190–310 piconewtons) as between proteins in antibody–antigen interactions (244 piconewtons). Quantitative measurements of adhesion forces between glycans from identical species versus glycans from different species confirmed the species specificity of the interaction. Glycan-coated beads aggregated according to their species of origin, i.e., the same way as live sponge cells did. Live cells also demonstrated species selective binding to glycans coated on surfaces. These findings confirm for the first time the existence of relatively strong and species-specific recognition between surface glycans, a process that may have significant implications in cellular recognition.en
dc.description.sponsorshipThis work was supported by the Friedrich Miescher Institute, branch of the Novartis Research Foundation, the M.E. Müller Foundation, and the Swiss National Research Foundationen
dc.publisherRockefeller University Pressen
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 Unported*
dc.subjectCell–cell recognitionen
dc.subjectCell surface proteoglycanen
dc.subjectCarbohydrate–carbohydrate interactionen
dc.subjectSpecies specificityen
dc.subjectAdhesion forceen
dc.titleCarbohydrate–carbohydrate interaction provides adhesion force and specificity for cellular recognitionen

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