Carbohydrate–carbohydrate interaction provides adhesion force and specificity for cellular recognition
Burger, Max M.
MetadataShow full item record
KeywordCell–cell recognition; Cell surface proteoglycan; Carbohydrate–carbohydrate interaction; Species specificity; Adhesion force
The adhesion force and specificity in the first experimental evidence for cell–cell recognition in the animal kingdom were assigned to marine sponge cell surface proteoglycans. However, the question whether the specificity resided in a protein or carbohydrate moiety could not yet be resolved. Here, the strength and species specificity of cell–cell recognition could be assigned to a direct carbohydrate–carbohydrate interaction. Atomic force microscopy measurements revealed equally strong adhesion forces between glycan molecules (190–310 piconewtons) as between proteins in antibody–antigen interactions (244 piconewtons). Quantitative measurements of adhesion forces between glycans from identical species versus glycans from different species confirmed the species specificity of the interaction. Glycan-coated beads aggregated according to their species of origin, i.e., the same way as live sponge cells did. Live cells also demonstrated species selective binding to glycans coated on surfaces. These findings confirm for the first time the existence of relatively strong and species-specific recognition between surface glycans, a process that may have significant implications in cellular recognition.
© 2004 Bucior et al. This article is distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. The definitive version was published in Journal of Cell Biology 165 (2004): 529-537, doi:10.1083/jcb.200309005.
Suggested CitationJournal of Cell Biology 165 (2004): 529-537
The following license files are associated with this item:
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 3.0 Unported
Showing items related by title, author, creator and subject.
Self-recognition and Ca2+-dependent carbohydrate–carbohydrate cell adhesion provide clues to the Cambrian explosion Fernandez-Busquets, Xavier; Kornig, Andre; Bucior, Iwona; Burger, Max M.; Anselmetti, Dario (2009-07-17)The Cambrian explosion of life was a relatively short period ca. 540 million years ago that marked a generalized acceleration in the evolution of most animal phyla, but the trigger of this key biological event remains ...
Accurate automatic quantification of taxa-specific plankton abundance using dual classification with correction Hu, Qiao; Davis, Cabell S. (Inter-Research, 2006-01-11)Optical imaging samplers are becoming widely used in plankton ecology, but image analysis methods have lagged behind image acquisition rates. Automated methods for analysis and recognition of plankton images have been ...
Zhang, Ying; Zagnitko, Olga; Rodionova, Irina; Osterman, Andrei; Godzik, Adam (Public Library of Science, 2011-12-22)Function diversification in large protein families is a major mechanism driving expansion of cellular networks, providing organisms with new metabolic capabilities and thus adding to their evolutionary success. However, ...