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dc.contributor.authorHutt, Karla J.  Concept link
dc.contributor.authorShi, Zhanquan  Concept link
dc.contributor.authorAlbertini, David F.  Concept link
dc.contributor.authorPetroff, Brian K.  Concept link
dc.date.accessioned2008-03-26T13:50:07Z
dc.date.available2008-03-26T13:50:07Z
dc.date.issued2008-01-02
dc.identifier.citationBMC Developmental Biology 8 (2008): 1en
dc.identifier.urihttps://hdl.handle.net/1912/2126
dc.description© 2008 Hutt et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License 2.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The definitive version was published in BMC Developmental Biology 8 (2008): 1, doi:10.1186/1471-213X-8-1.en
dc.description.abstractEnvironmental toxicants, whose actions are often mediated through the aryl hydrocarbon receptor (AhR) pathway, pose risks to the health and well-being of exposed species, including humans. Of particular concern are exposures during the earliest stages of development that while failing to abrogate embryogenesis, may have long term effects on newborns or adults. The purpose of this study was to evaluate the effect of maternal exposure to the AhR-specific ligand 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on the development of rat pre-implantation embryos with respect to nuclear and cytoskeletal architecture and cell lineage allocation. We performed a systematic 3 dimensional (3D) confocal microscopy analysis of rat pre-implantation embryos following maternal exposure to environmentally relevant doses of TCDD. Both chronic (50 ng/kg/wk for 3 months) and acute (50 ng/kg and 1 μg/kg at proestrus) maternal TCDD exposure disrupted morphogenesis at the compaction stage (8–16 cell), with defects including monopolar spindle formation, f-actin capping and fragmentation due to aberrant cytokinesis. Additionally, the size, shape and position of nuclei were modified in compaction stage pre-implantation embryos collected from treated animals. Notably, maternal TCDD exposure did not compromise survival to blastocyst, which with the exception of nuclear shape, were morphologically similar to control blastocysts. We have identified the compaction stage of pre-implantation embryogenesis as critically sensitive to the effects of TCDD, while survival to the blastocyst stage is not compromised. To the best of our knowledge this is the first in vivo study to demonstrate a critical window of pre-implantation mammalian development that is vulnerable to disruption by an AhR ligand at environmentally relevant doses.en
dc.description.sponsorshipThis research was supported by NIH/NIEHS-012916 (BKP), ESHE Fund (DFA), Hall Family Foundation (DFA and KJH) and Biomedical Research Training Grant KUMC (KJH).en
dc.format.mimetypevideo/quicktime
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen
dc.publisherBioMed Centralen
dc.relation.urihttps://doi.org/10.1186/1471-213X-8-1
dc.rightsAttribution 2.0 Generic*
dc.rights.urihttp://creativecommons.org/licenses/by/2.0*
dc.titleThe environmental toxicant 2,3,7,8-tetrachlorodibenzo-p-dioxin disrupts morphogenesis of the rat pre-implantation embryoen
dc.typeArticleen
dc.identifier.doi10.1186/1471-213X-8-1


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