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dc.contributor.authorBest, Aaron A.  Concept link
dc.contributor.authorMorrison, Hilary G.  Concept link
dc.contributor.authorMcArthur, Andrew G.  Concept link
dc.contributor.authorSogin, Mitchell L.  Concept link
dc.contributor.authorOlsen, Gary J.  Concept link
dc.date.accessioned2005-11-28T21:38:49Z
dc.date.available2005-11-28T21:38:49Z
dc.date.issued2004
dc.identifier.citationGenome Research 14 (2004): 1537-1547en
dc.identifier.urihttps://hdl.handle.net/1912/210
dc.descriptionAuthor Posting. © Cold Spring Harbor Laboratory Press, 2004. This article is posted here by permission of Cold Spring Harbor Laboratory Press for personal use, not for redistribution. The definitive version was published in Genome Research 14 (2004): 1537-1547, doi:10.1101/gr.2256604.
dc.description.abstractThe Giardia lamblia genome sequencing project affords us a unique opportunity to conduct comparative analyses of core cellular systems between early and late-diverging eukaryotes on a genome-wide scale. We report a survey to identify canonical transcription components in Giardia, focusing on RNA polymerase (RNAP) subunits and transcription-initiation factors. Our survey revealed that Giardia contains homologs to 21 of the 28 polypeptides comprising eukaryal RNAPI, RNAPII, and RNAPIII; six of the seven RNAP subunits without giardial homologs are polymerase specific. Components of only four of the 12 general transcription initiation factors have giardial homologs. Surprisingly, giardial TATA-binding protein (TBP) is highly divergent with respect to archaeal and higher eukaryotic TBPs, and a giardial homolog of transcription factor IIB was not identified. We conclude that Giardia represents a transition during the evolution of eukaryal transcription systems, exhibiting a relatively complete set of RNAP subunits and a rudimentary basal initiation apparatus for each transcription system. Most class-specific RNAP subunits and basal initiation factors appear to have evolved after the divergence of Giardia from the main eukaryotic line of descent. Consequently, Giardia is predicted to be unique in many aspects of transcription initiation with respect to paradigms derived from studies in crown eukaryotes.en
dc.description.sponsorshipThis work was supported in part by NIH grant AI43273 to M.L.S., by NIH grant AI51089 to A.G.M, and DOE grant DE-FG02-01ER63201 to G.J.O. Additional support was provided by the G. Unger Vetlesen Foundation and LI-COR Biotechnology.en
dc.format.extent243197 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen
dc.publisherCold Spring Harbor Laboratory Pressen
dc.relation.urihttps://doi.org/10.1101/gr.2256604
dc.subjectGiardia lambliaen
dc.subjectEukaryal transcription systemsen
dc.titleEvolution of eukaryotic transcription : insights from the genome of Giardia lambliaen
dc.typeArticleen
dc.identifier.doi10.1101/gr.2256604


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