A non-invasive method for measuring preimplantation embryo physiology
Trimarchi, James R.
Porterfield, D. Marshall
Smith, Peter J. S.
Keefe, David L.
MetadataShow full item record
The physiology of the early embryo may be indicative of embryo vitality and therefore methods for non-invasively monitoring physiological parameters from embryos could improve preimplantation diagnoses. The self-referencing electrophysiological technique is capable of non-invasive measurement of the physiology of individual cells by monitoring the movement of ions and molecules between the cell and the surrounding media. Here we use this technique to monitor gradients of calcium, potassium, oxygen and hydrogen peroxide around individual mouse preimplantation embryos. The calcium-sensitive electrode in self-referencing mode identified a region of elevated calcium concentration ([similar]0.25 pmol) surrounding each embryo. The calcium gradient surrounding embryos was relatively steep, such that the region of elevated calcium extended into the medium only 4 [mu]m from the embryo. By contrast, using an oxygen-sensitive electrode an extensive gradient of reduced dissolved oxygen concentration was measured surrounding the embryo and extended tens of micrometres into the medium. A gradient of neither potassium nor hydrogen peroxide was observed around unperturbed embryos. We also demonstrate that monitoring the physiology of embryos using the self-referencing technique does not compromise their subsequent development. Blastocysts studied with the self-referencing technique implanted and developed to term at the same frequency as did unexamined, control embryos. Therefore, the self-referencing electrode provides a valuable non-invasive technique for studying the physiology and pathophysiology of individual embryos without hindering their subsequent development.
Author Posting. © Cambridge University Press, 2000. This article is posted here by permission of Cambridge University Press for personal use, not for redistribution. The definitive version was published in Zygote 8 (2000): 15-24, doi:10.1017/S0967199400000782.
Suggested CitationZygote 8 (2000): 15-24
Showing items related by title, author, creator and subject.
Physiological and behavioral responses, and their variability, in squid, Doryteuthis pealeii, embryos and paralarvae reared under chronic ocean acidification Zakroff, Casey (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 2019-09)Ocean acidification (OA) and related stressors, like warming, are occurring rapidly in coastal systems. There is concern about the impacts these stressors may have on the early development of species that use the nearshore ...
The role of Nrf1 and Nrf2 in the regulation of glutathione and redox dynamics in the developing zebrafish embryo Sant, Karilyn E.; Hansen, Jason M.; Williams, Larissa M.; Tran, Nancy L.; Goldstone, Jared V.; Stegeman, John J.; Hahn, Mark E.; Timme-Laragy, Alicia R. (Elsevier, 2017-05-30)Redox signaling is important for embryogenesis, guiding pathways that govern processes crucial for embryo patterning, including cell polarization, proliferation, and apoptosis. Exposure to pro-oxidants during this period ...
Brun, Nadja R.; Koch, Bjørn E. V.; Varela, Mónica; Peijnenburg, Willie J. G. M.; Spaink, Herman P.; Vijver, Martina G. (2018-03)Major molecular mechanisms that underpin the toxicity of nanoparticles (NPs) are the formation of reactive oxygen species and the induction of inflammation. The latter is frequently observed in vitro and in mammalian ...