Show simple item record

dc.contributor.authorDumollard, Remi  Concept link
dc.contributor.authorHammar, Katherine M.  Concept link
dc.contributor.authorPorterfield, D. Marshall  Concept link
dc.contributor.authorSmith, Peter J. S.  Concept link
dc.contributor.authorCibert, Christian  Concept link
dc.contributor.authorRouviere, Christian  Concept link
dc.contributor.authorSardet, Christian  Concept link
dc.date.accessioned2005-11-25T20:37:17Z
dc.date.available2005-11-25T20:37:17Z
dc.date.issued2003
dc.identifier.citationDevelopment 130 (2003): 683-692en
dc.identifier.urihttps://hdl.handle.net/1912/206
dc.descriptionAuthor Posting. © Company of Biologists Limited, 2003. This article is posted here by permission of Company of Biologists Limited for personal use, not for redistribution. The definitive version was published in Development 130 (2003): 683-692, doi:10.1242/10.1242/dev.00296.
dc.description.abstractFertilization increases both cytosolic Ca2+ concentration and oxygen consumption in the egg but the relationship between these two phenomena remains largely obscure. We have measured mitochondrial oxygen consumption and the mitochondrial NADH concentration on single ascidian eggs and found that they increase in phase with each series of meiotic Ca2+ waves emitted by two pacemakers (PM1 and PM2). Oxygen consumption also increases in response to Ins(1,4,5)P3-induced Ca2+ transients. Using mitochondrial inhibitors we show that active mitochondria sequester cytosolic Ca2+ during sperm-triggered Ca2+ waves and that they are strictly necessary for triggering and sustaining the activity of the meiotic Ca2+ wave pacemaker PM2. Strikingly, the activity of the Ca2+ wave pacemaker PM2 can be restored or stimulated by flash photolysis of caged ATP. Taken together our observations provide the first evidence that, in addition to buffering cytosolic Ca2+, the egg's mitochondria are stimulated by Ins(1,4,5)P3-mediated Ca2+ signals. In turn, mitochondrial ATP production is required to sustain the activity of the meiotic Ca2+ wave pacemaker PM2.en
dc.description.sponsorshipThis work was supported by AFM and ARC grants to C. S.en
dc.format.extent476357 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoenen
dc.publisherCompany of Biologists Limiteden
dc.relation.urihttps://doi.org/10.1242/dev.00296
dc.subjectFertilizationen
dc.subjectRespirationen
dc.subjectCa2+ wavesen
dc.subjectMitochondriaen
dc.subjectEndoplasmic reticulumen
dc.subjectATPen
dc.subjectCa2+ wave pacemakersen
dc.subjectAscidianen
dc.titleMitochondrial respiration and Ca2+ waves are linked during fertilization and meiosis completionen
dc.typeArticleen
dc.identifier.doi10.1242/dev.00296


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record