Putative ammonia-oxidizing Crenarchaeota in suboxic waters of the Black Sea : a basin-wide ecological study using 16S ribosomal and functional genes and membrane lipids
Coolen, Marco J. L.
van Bleijswijk, Judith
Hopmans, Ellen C.
Kuypers, Marcel M. M.
Wakeham, Stuart G.
Sinninghe Damste, Jaap S.
MetadataShow full item record
Within the upper 400 m at western, central, and eastern stations in the world’s largest stratified basin, the Black Sea, we studied the qualitative and quantitative distribution of putative nitrifying Archaea based on their genetic markers (16S rDNA, amoA encoding for the alfa-subunit of archaeal ammonia monooxygenase), and crenarchaeol, the specific glycerol diphytanyl glycerol tetraether (GDGT) of pelagic Crenarchaeota within the Group I.1a. Marine Crenarchaeota were the most abundant Archaea (up to 98% of the total archaeal 16S rDNA copies) in the suboxic layers with oxygen levels as low as 1 μM including layers where previously anammox bacteria were described (Kuypers et al., 2003). Different marine crenarchaeotal phylotypes (both 16S rDNA and amoA) were found at the upper part of the suboxic zone as compared to the base of the suboxic zone and the upper 15-30 m of the anoxic waters with prevailing sulfide concentrations of up to 30 μM. Crenarchaeol concentrations were higher in the sulfidic chemocline as compared to the suboxic zone. These results indicate an abundance of putative nitrifying Archaea at very low oxygen levels within the Black Sea and might form an important source of nitrite for the anammox reaction.
Author Posting. © Blackwell, 2007. This is the author's version of the work. It is posted here by permission of Blackwell for personal use, not for redistribution. The definitive version was published in Environmental Microbiology 9 (2007): 1001-1016, doi:10.1111/j.1462-2920.2006.01227.x.
Showing items related by title, author, creator and subject.
Temporal and spatial variability in sedimentation in the Black Sea : cruise report R/V Knorr 134-8, Black Sea Leg 1, April 16-May 7, 1988 Honjo, Susumu; Hay, Bernward J.; Members of the Scientific Shipboard Party (Woods Hole Oceanographic Institution, 1988-10)This document represents the cruise report of the highly successful Leg 1 of the R/V Knorr cruise to the Black Sea (Cruise 134-8) as a joint Turkish-American Oceanographic Expedition (Izmir to Istanbul, April 16 to May ...
Differential responses of ammonia-oxidizing archaea and bacteria to long-term fertilization in a New England salt marsh Peng, Xuefeng; Yando, Erik; Hildebrand, Erica; Dwyer, Courtney; Kearney, Anne; Waciega, Alex; Valiela, Ivan; Bernhard, Anne E. (Frontiers Media, 2013-01-22)Since the discovery of ammonia-oxidizing archaea (AOA), new questions have arisen about population and community dynamics and potential interactions between AOA and ammonia-oxidizing bacteria (AOB). We investigated the ...
Enrichment and characterization of ammonia-oxidizing archaea from the open ocean : phylogeny, physiology and stable isotope fractionation Santoro, Alyson E.; Casciotti, Karen L. (2011-03-14)Archaeal genes for ammonia oxidation are widespread in the marine environment, but direct physiological evidence for ammonia oxidation by marine archaea is limited. We report the enrichment and characterization of three ...