Coexistence of multiple proteobacterial endosymbionts in the gills of the wood-boring bivalve Lyrodus pedicellatus (Bivalvia: Teredinidae)
MetadataShow full item record
Wood-boring bivalves of the family Teredinidae (commonly called shipworms) are known to harbor dense populations of gram-negative bacteria within specialized cells (bacteriocytes) in their gills. These symbionts are thought to provide enzymes, e.g., cellulase and dinitrogenase, which assist the host in utilizing wood as a primary food source. A cellulolytic, dinitrogen-fixing bacterium, Teredinibacter turnerae, has been isolated from the gill tissues of numerous teredinid bivalves and has been proposed to constitute the sole or predominant symbiont of this bivalve family. Here we demonstrate that one teredinid species, Lyrodus pedicellatus, contains at least four distinct bacterial 16S rRNA types within its gill bacteriocytes, one of which is identical to that of T. turnerae. Phylogenetic analyses indicate that the three newly detected ribotypes are derived from gamma proteobacteria that are related to but distinct (>6.5% sequence divergence) from T. turnerae. In situ hybridizations with 16S rRNA-directed probes demonstrated that the pattern of occurrence of symbiont ribotypes within bacteriocytes was predictable and specific, with some bacteriocytes containing two symbiont ribotypes. However, only two of the six possible pairwise combinations of the four ribotypes were observed to cooccur within the same host cells. The results presented here are consistent with the existence of a complex multiple symbiosis in this shipworm species.
Author Posting. © American Society for Microbiology, 2002. This article is posted here by permission of American Society for Microbiology for personal use, not for redistribution. The definitive version was published in [citation], doi:10.1128/AEM.68.12.6292-6299.2002.
Showing items related by title, author, creator and subject.
Allen, J. A. (Cambridge University Press, 2000-10-31)The shell and internal anatomy of the montacutid bivalve Mysella verrilli is described for the first time. The species is remarkable in that the oesophagus has developed into a suctorial proboscis. This has been accompanied ...
Reproduction among protobranch bivalves of the family Nuculidae from sublittoral, bathyal, and abyssal depths off the New England coast of North America Scheltema, Rudolf S.; Williams, Isabelle P. (2009-02-04)Protobranch bivalve species of the family Nuculidae pass either through a planktonic lecithotrophic larval stage or a direct non-planktonic development. Oogenesis of the three sublittoral species examined is synchronous. ...
Bivalves as indicators of environmental variation and potential anthropogenic impacts in the southern Barents Sea Carroll, Michael L.; Johnson, Beverly J.; Henkes, Gregory A.; McMahon, Kelton W.; Voronkov, Andrey; Ambrose, William G.; Denisenko, Stanislav G. (2009-04)Identifying patterns and drivers of natural variability in populations is necessary to gauge potential effects of climatic change and the expected increases in commercial activities in the Arctic on communities and ...