• Login
    About WHOAS
    View Item 
    •   WHOAS Home
    • Woods Hole Oceanographic Institution
    • Biology
    • View Item
    •   WHOAS Home
    • Woods Hole Oceanographic Institution
    • Biology
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of WHOASCommunities & CollectionsBy Issue DateAuthorsTitlesKeywordsThis CollectionBy Issue DateAuthorsTitlesKeywords

    My Account

    LoginRegister

    Statistics

    View Usage Statistics

    Diversity and dynamics of a North Atlantic coastal Vibrio community

    Thumbnail
    View/Open
    Thompson Diversity.pdf (587.0Kb)
    Date
    2004-07
    Author
    Thompson, Janelle R.  Concept link
    Randa, Mark A.  Concept link
    Marcelino, Luisa A.  Concept link
    Tomita-Mitchell, Aoy  Concept link
    Lim, Ee Lin  Concept link
    Polz, Martin F.  Concept link
    Metadata
    Show full item record
    Citable URI
    https://hdl.handle.net/1912/196
    As published
    https://doi.org/10.1128/AEM.70.7.4103-4110.2004
    DOI
    10.1128/AEM.70.7.4103-4110.2004
    Keyword
     Vibrios; Marine bacteria 
    Abstract
    Vibrios are ubiquitous marine bacteria that have long served as models for heterotrophic processes and have received renewed attention because of the discovery of increasing numbers of facultatively pathogenic strains. Because the occurrence of specific vibrios has frequently been linked to the temperature, salinity, and nutrient status of water, we hypothesized that seasonal changes in coastal water bodies lead to distinct vibrio communities and sought to characterize their level of differentiation. A novel technique was used to quantify shifts in 16S rRNA gene abundance in samples from Barnegat Bay, N.J., collected over a 15-month period. Quantitative PCR (QPCR) with primers specific for the genus Vibrio was combined with separation and quantification of amplicons by constant denaturant capillary electrophoresis (CDCE). Vibrio populations identified by QPCR-CDCE varied between summer and winter samples, suggesting distinct warm-water and year-round populations. Identification of the CDCE populations by cloning and sequencing of 16S rRNA genes from two summer and two winter samples confirmed this distinction. It further showed that CDCE populations corresponded in most cases to ~98% rRNA similarity groups and suggested that the abundance of these follows temperature trends. Phylogenetic comparison yielded closely related cultured and often pathogenic representatives for most sequences, and the temperature ranges of these isolates confirmed the trends seen in the environmental samples. Overall, this suggests that temperature is a good predictor of the occurrence of closely related vibrios but that considerable microdiversity of unknown significance coexists within this trend.
    Description
    Author Posting. © American Society for Microbiology, 2004. This article is posted here by permission of American Society for Microbiology for personal use, not for redistribution. The definitive version was published in Applied and Environmental Microbiology 70 (2004): 4103-4110, doi:10.1128/AEM.70.7.4103-4110.2004.
    Collections
    • Biology
    Suggested Citation
    Applied and Environmental Microbiology 70 (2004): 4103-4110
     

    Related items

    Showing items related by title, author, creator and subject.

    • Thumbnail

      The quorum-sensing regulation of Vibrio fischeri : novel components of the autoinducer/LuxR regulatory circuit 

      Callahan, Sean M. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 1999-06)
      In the marine bacterium Vibrio fischeri two intercellular homoserine-Iactone signal molecules (luxI-dependent 30C6-HSL and the ainS-dependent C8-HSL) and the transcriptional activator LuxR regulate the luminescence system ...
    • Thumbnail

      Vibrio elicits targeted transcriptional responses from copepod hosts 

      Almada, Amalia A.; Tarrant, Ann M. (2016-05)
      Copepods are abundant crustaceans that harbor diverse bacterial communities, yet the nature of their interactions with microbiota are poorly understood . Here, we report that Vibrio elicits targeted transcriptional ...
    • Thumbnail

      Supplementary Information: Antibiotic resistance in Vibrio-like bacteria is common on Cape Cod, MA beaches 

      May, Megan K.; Gast, Rebecca J. (2018-10-23)
      Antibiotic resistance (AR) is a natural process, enhanced by anthropogenic antibiotic use. Natural environments, like the ocean, act as reservoirs of resistance; but until recently, little research has examined their ...
    All Items in WHOAS are protected by original copyright, with all rights reserved, unless otherwise indicated. WHOAS also supports the use of the Creative Commons licenses for original content.
    A service of the MBLWHOI Library | About WHOAS
    Contact Us | Send Feedback | Privacy Policy
    Core Trust Logo