Characterization of Trichodesmium spp. by genetic techniques
Orcutt, K. M.
Webb, Eric A.
Waterbury, John B.
MetadataShow full item record
KeywordTrichodesmium spp.; Highly iterated palindrome (HIP1) fingerprinting; Denaturing gradient gel electrophoresis (DGGE); Internal transcribed spacer (ITS)
The genetic diversity of Trichodesmium spp. from natural populations (off Bermuda in the Sargasso Sea and off North Australia in the Arafura and Coral Seas) and of culture isolates from two regions (Sargasso Sea and Indian Ocean) was investigated. Three independent techniques were used, including a DNA fingerprinting method based on a highly iterated palindrome (HIP1), denaturing gradient gel electrophoresis of a hetR fragment, and sequencing of the internal transcribed spacer (ITS) of the 16S-23S rDNA region. Low genetic diversity was observed in natural populations of Trichodesmium spp. from the two hemispheres. Culture isolates of Trichodesmium thiebautii, Trichodesmium hildebrandtii, Trichodesmium tenue, and Katagnymene spiralis displayed remarkable similarity when these techniques were used, suggesting that K. spiralis is very closely related to the genus Trichodesmium. The largest genetic variation was found between Trichodesmium erythraeum and all other species of Trichodesmium, including a species of Katagnymene. Our data obtained with all three techniques suggest that there are two major clades of Trichodesmium spp. The HIP1 fingerprinting and ITS sequence analyses allowed the closely related species to be distinguished. This is the first report of the presence of HIP1 in marine cyanobacteria.
Author Posting. © American Society for Microbiology, 2002. This article is posted here by permission of American Society for Microbiology for personal use, not for redistribution. The definitive version was published in Applied and Environmental Microbiology 68 (2002): 2236-2245, doi:10.1128/AEM.68.5.2236-2245.2002.
Showing items related by title, author, creator and subject.
Iron stress in open-ocean cyanobacteria (Synechococcus, Trichodesmium, and Crocosphaera spp.) : identification of the IdiA protein Webb, Eric A.; Moffett, James W.; Waterbury, John B. (American Society for Microbiology, 2001-12)Cyanobacteria are prominent constituents of the marine biosphere that account for a significant percentage of oceanic primary productivity. In an effort to resolve how open-ocean cyanobacteria persist in regions where the ...
Overexpression and characterization of an iron storage and DNA-binding Dps protein from Trichodesmium erythraeum Castruita, M.; Saito, Mak A.; Schottel, P. C.; Elmegreen, L. A.; Myneni, Satish C. B.; Stiefel, E. I.; Morel, Francois M. M. (American Society for Microbiology, 2006-04)Although the role of iron in marine productivity has received a great deal of attention, no iron storage protein has been isolated from a marine microorganism previously. We describe an Fe-binding protein belonging to the ...
Microbial interactions associated with biofilms attached to Trichodesmium spp. and detrital particles in the ocean Hmelo, Laura R. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 2010-06)Quorum sensing (QS) via acylated homoserine lactones (AHLs) was discovered in the ocean, yet little is known about its role in the ocean beyond its involvement in certain symbiotic interactions. The objectives of this ...