Characterization of protistan assemblages in the Ross Sea, Antarctica, by denaturing gradient gel electrophoresis


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dc.contributor.author Gast, Rebecca J.
dc.contributor.author Dennett, Mark R.
dc.contributor.author Caron, David A.
dc.date.accessioned 2005-11-25T13:31:38Z
dc.date.available 2005-11-25T13:31:38Z
dc.date.issued 2004-04
dc.identifier.citation Applied and Environmental Microbiology 70 (2004): 2028-2037 en
dc.identifier.uri http://hdl.handle.net/1912/192
dc.description Author Posting. © American Society for Microbiology, 2004. This article is posted here by permission of American Society for Microbiology for personal use, not for redistribution. The definitive version was published in Applied and Environmental Microbiology 70 (2004): 2028-2037, doi:10.1128/AEM.70.4.2028-2037.2004.
dc.description.abstract The diversity of protistan assemblages has traditionally been studied using microscopy and morphological characterization, but these methods are often inadequate for ecological studies of these communities because most small protists inherently lack adequate taxonomic characters to facilitate their identification at the species level and many protistan species also do not preserve well. We have therefore used a culture-independent approach (denaturing gradient gel electrophoresis [DGGE]) to obtain an assessment of the genetic composition and distribution of protists within different microhabitats (seawater, meltwater or slush on sea-ice floes, and ice) of the Ross Sea, Antarctica. Samples of the same type (e.g., water) shared more of the same bands than samples of different types (e.g., ice versus water), despite being collected from different sites. These findings imply that samples from the same environment have a similar protistan species composition and that the type of microenvironment significantly influences the protistan species composition of these Antarctic assemblages. It should be noted that a large number of bands among the samples within each microhabitat were distinct, indicating the potential presence of significant genetic diversity within each microenvironment. Sequence analysis of selected DGGE bands revealed sequences that represent diatoms, dinoflagellates, ciliates, flagellates, and several unidentified eukaryotes. en
dc.description.sponsorship This work was supported by National Science Foundation grants OPP-9714299 and OPP-0125437. en
dc.format.extent 801630 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US en
dc.publisher American Society for Microbiology en
dc.relation.uri http://dx.doi.org/10.1128/AEM.70.4.2028-2037.2004
dc.subject Protistan assemblages en
dc.subject Denaturing gradient gel electrophoresis (DGGE) en
dc.title Characterization of protistan assemblages in the Ross Sea, Antarctica, by denaturing gradient gel electrophoresis en
dc.type Article en
dc.identifier.doi 10.1128/AEM.70.4.2028-2037.2004

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