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    Development and application of a monoclonal-antibody technique for counting Aureococcus anophagefferens, an alga causing recurrent brown tides in the Mid-Atlantic United States

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    Caron Development.pdf (3.230Mb)
    Date
    2003-09
    Author
    Caron, David A.  Concept link
    Dennett, Mark R.  Concept link
    Moran, Dawn M.  Concept link
    Schaffner, Rebecca A.  Concept link
    Lonsdale, Darcy J.  Concept link
    Gobler, Christopher J.  Concept link
    Nuzzi, Robert  Concept link
    McLean, Tim I.  Concept link
    Metadata
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    Citable URI
    https://hdl.handle.net/1912/187
    As published
    https://doi.org/10.1128/AEM.69.9.5492-5502.2003
    DOI
    10.1128/AEM.69.9.5492-5502.2003
    Keyword
     Aureococcus anophagefferens; Harmful algal blooms (HAB); Monoclonal antibody (MAb) 
    Abstract
    A method was developed for the rapid detection and enumeration of Aureococcus anophagefferens, the cause of harmful algal blooms called "brown tides" in estuaries of the Mid-Atlantic United States. The method employs a monoclonal antibody (MAb) and a colorimetric, enzyme-linked immunosorbent assay format. The MAb obtained exhibits high reactivity with A. anophagefferens and very low cross-reactivities with a phylogenetically diverse array of other protists and bacteria. Standard curves are constructed for each 96-well microtiter plate by using known amounts of a preserved culture of A. anophagefferens. This approach allows estimation of the abundance of the alga in natural samples. The MAb method was compared to an existing method that employs polyclonal antibodies and epifluorescence microscopy and to direct microscopic counts of A. anophagefferens in samples with high abundances of the alga. The MAb method provided increased quantitative accuracy and greatly reduced sample processing time. A spatial survey of several Long Island estuaries in May 2000 using this new approach documented a range of abundances of A. anophagefferens in these bays spanning nearly 3 orders of magnitude.
    Description
    Author Posting. © American Society for Microbiology, 2003. This article is posted here by permission of American Society for Microbiology for personal use, not for redistribution. The definitive version was published in Applied and Environmental Microbiology 69 (2003): 5492-5502, doi:10.1128/AEM.69.9.5492-5502.2003.
    Collections
    • Biology
    Suggested Citation
    Article: Caron, David A., Dennett, Mark R., Moran, Dawn M., Schaffner, Rebecca A., Lonsdale, Darcy J., Gobler, Christopher J., Nuzzi, Robert, McLean, Tim I., "Development and application of a monoclonal-antibody technique for counting Aureococcus anophagefferens, an alga causing recurrent brown tides in the Mid-Atlantic United States", Applied and Environmental Microbiology 69 (2003): 5492-5502, DOI:10.1128/AEM.69.9.5492-5502.2003, https://hdl.handle.net/1912/187
     

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