Protein folding failure sets high-temperature limit on growth of phage P22 in Salmonella enterica serovar Typhimurium
Pope, Welkin H.
MetadataShow full item record
The high-temperature limit for growth of microorganisms differs greatly depending on their species and habitat. The importance of an organism's ability to manage thermal stress is reflected in the ubiquitous distribution of the heat shock chaperones. Although many chaperones function to reduce protein folding defects, it has been difficult to identify the specific protein folding pathways that set the high-temperature limit of growth for a given microorganism. We have investigated this for a simple system, phage P22 infection of Salmonella enterica serovar Typhimurium. Production of infectious particles exhibited a broad maximum of 150 phage per cell when host cells were grown at between 30 and 39°C in minimal medium. Production of infectious phage declined sharply in the range of 40 to 41°C, and at 42°C, production had fallen to less than 1% of the maximum rate. The host cells maintained optimal division rates at these temperatures. The decrease in phage infectivity was steeper than the loss of physical particles, suggesting that noninfectious particles were formed at higher temperatures. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed a decrease in the tailspike adhesins assembled on phage particles purified from cultures incubated at higher temperatures. The infectivity of these particles was restored by in vitro incubation with soluble tailspike trimers. Examination of tailspike folding and assembly in lysates of phage-infected cells confirmed that the fraction of polypeptide chains able to reach the native state in vivo decreased with increasing temperature, indicating a thermal folding defect rather than a particle assembly defect. Thus, we believe that the folding pathway of the tailspike adhesin sets the high-temperature limit for P22 formation in Salmonella serovar Typhimurium.
Author Posting. © American Society for Microbiology, 2004. This article is posted here by permission of American Society for Microbiology for personal use, not for redistribution. The definitive version was published in Applied and Environmental Microbiology 70 (2004): 4840-4847, doi:10.1128/AEM.70.8.4840-4847.2004.
Showing items related by title, author, creator and subject.
Davids, Barbara J.; Reiner, David S.; Birkeland, Shanda R.; Preheim, Sarah P.; Cipriano, Michael J.; McArthur, Andrew G.; Gillin, Frances D. (Public Library of Science, 2006-12)Since the Giardia lamblia cyst wall is necessary for survival in the environment and host infection, we tested the hypothesis that it contains proteins other than the three known cyst wall proteins. Serial analysis of gene ...
Primary production of particulate protein amino acids : algal protein metabolism and its relationship to the composition of particulate organic matter Lohrenz, Steven E. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 1985-04)The biochemical and physiological bases underlying primary production of particulate protein amino acids were investigated in an effort to understand the relationship between algal protein metabolism and particulate organic ...
Squid giant axon contains neurofilament protein mRNA but does not synthesize neurofilament proteins Gainer, Harold; House, Shirley; Kim, Dong Sun; Chin, Hemin; Pant, Harish C. (2016-05-05)When isolated squid giant axons are incubated in radioactive amino acids, abundant newly synthesized proteins are found in the axoplasm. These proteins are translated in the adaxonal Schwann cells and subsequently transferred ...