Comparative analysis of methane-oxidizing archaea and sulfate-reducing bacteria in anoxic marine sediments
Orphan, V. J.
Paull, Charles K.
Taylor, L. T.
Sylva, Sean P.
Hayes, John M.
DeLong, Edward F.
MetadataShow full item record
The oxidation of methane in anoxic marine sediments is thought to be mediated by a consortium of methane-consuming archaea and sulfate-reducing bacteria. In this study, we compared results of rRNA gene (rDNA) surveys and lipid analyses of archaea and bacteria associated with methane seep sediments from several different sites on the Californian continental margin. Two distinct archaeal lineages (ANME-1 and ANME-2), peripherally related to the order Methanosarcinales, were consistently associated with methane seep marine sediments. The same sediments contained abundant 13C-depleted archaeal lipids, indicating that one or both of these archaeal groups are members of anaerobic methane-oxidizing consortia. 13C-depleted lipids and the signature 16S rDNAs for these archaeal groups were absent in nearby control sediments. Concurrent surveys of bacterial rDNAs revealed a predominance of delta -proteobacteria, in particular, close relatives of Desulfosarcina variabilis. Biomarker analyses of the same sediments showed bacterial fatty acids with strong 13C depletion that are likely products of these sulfate-reducing bacteria. Consistent with these observations, whole-cell fluorescent in situ hybridization revealed aggregations of ANME-2 archaea and sulfate-reducing Desulfosarcina and Desulfococcus species. Additionally, the presence of abundant 13C-depleted ether lipids, presumed to be of bacterial origin but unrelated to ether lipids of members of the order Desulfosarcinales, suggests the participation of additional bacterial groups in the methane-oxidizing process. Although the Desulfosarcinales and ANME-2 consortia appear to participate in the anaerobic oxidation of methane in marine sediments, our data suggest that other bacteria and archaea are also involved in methane oxidation in these environments.
Author Posting. © American Society for Microbiology, 2001. This article is posted here by permission of American Society for Microbiology for personal use, not for redistribution. The definitive version was published in Applied and Environmental Microbiology 67 (2001): 1922-1934, doi:10.1128/AEM.67.4.1922-1934.2001.
Suggested CitationArticle: Orphan, V. J., Hinrichs, Kai-Uwe, Ussler, William, Paull, Charles K., Taylor, L. T., Sylva, Sean P., Hayes, John M., DeLong, Edward F., "Comparative analysis of methane-oxidizing archaea and sulfate-reducing bacteria in anoxic marine sediments", Applied and Environmental Microbiology 67 (2001): 1922-1934, DOI:10.1128/AEM.67.4.1922-1934.2001, https://hdl.handle.net/1912/183
Showing items related by title, author, creator and subject.
Dhillon, Ashita; Teske, Andreas; Dillon, Jesse; Stahl, David A.; Sogin, Mitchell L. (American Society for Microbiology, 2003-05)The Guaymas Basin (Gulf of California) is a hydrothermal vent site where thermal alteration of deposited planktonic and terrestrial organic matter forms petroliferous material which supports diverse sulfate-reducing bacteria. ...
Sulfate reduction and possible aerobic metabolism of the sulfate-reducing bacterium Desulfovibrio oxyclinae in a chemostat coculture with Marinobacter sp. strain MB under exposure to increasing oxygen concentrations Sigalevich, Pavel; Baev, Mark V.; Teske, Andreas; Cohen, Yehuda (American Society for Microbiology, 2000-11)A chemostat coculture of the sulfate-reducing bacterium Desulfovibrio oxyclinae together with a facultative aerobe heterotroph tentatively identified as Marinobacter sp. strain MB was grown under anaerobic conditions and ...
Modeling sulfate reduction in methane hydrate-bearing continental margin sediments : does a sulfate-methane transition require anaerobic oxidation of methane? Malinverno, Alberto; Pohlman, John W. (American Geophysical Union, 2011-07-12)The sulfate-methane transition (SMT), a biogeochemical zone where sulfate and methane are metabolized, is commonly observed at shallow depths (1–30 mbsf) in methane-bearing marine sediments. Two processes consume sulfate ...