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dc.contributor.authorNoel, Christophe  Concept link
dc.contributor.authorDufernez, Fabienne  Concept link
dc.contributor.authorGerbod, Delphine  Concept link
dc.contributor.authorEdgcomb, Virginia P.  Concept link
dc.contributor.authorDelgado-Viscogliosi, Pilar  Concept link
dc.contributor.authorHo, Lip-Chuen  Concept link
dc.contributor.authorSingh, Mulkit  Concept link
dc.contributor.authorWintjens, Rene  Concept link
dc.contributor.authorSogin, Mitchell L.  Concept link
dc.contributor.authorCapron, Monique  Concept link
dc.contributor.authorPierce, Raymond  Concept link
dc.contributor.authorZenner, Lionel  Concept link
dc.contributor.authorViscogliosi, Eric  Concept link
dc.date.accessioned2005-11-23T16:02:33Z
dc.date.available2005-11-23T16:02:33Z
dc.date.issued2005-01
dc.identifier.citationJournal of Clinical Microbiology 43 (2005): 348-355en
dc.identifier.urihttps://hdl.handle.net/1912/182
dc.descriptionAuthor Posting. © American Society for Microbiology, 2005. This article is posted here by permission of American Society for Microbiology for personal use, not for redistribution. The definitive version was published in Journal of Clinical Microbiology 43 (2005): 348-355, doi:10.1128/JCM.43.1.348-355.2005.
dc.description.abstractSmall-subunit (SSU) rRNA gene sequences were obtained by PCR from 12 Blastocystis isolates from humans, rats, and reptiles for which elongation factor 1{alpha} (EF-1{alpha}) gene sequences are already available. These new sequences were analyzed by the Bayesian method in a broad phylogeny including, for the first time, all Blastocystis sequences available in the databases. Phylogenetic trees identified seven well-resolved groups plus several discrete lineages that could represent newly defined clades. Comparative analysis of SSU rRNA- and EF-1{alpha}-based trees obtained by maximum-likelihood methods from a restricted sampling (13 isolates) revealed overall agreement between the two phylogenies. In spite of their morphological similarity, sequence divergence among Blastocystis isolates reflected considerable genetic diversity that could be correlated with the existence of potentially ≥12 different species within the genus. Based on this analysis and previous PCR-based genotype classification data, six of these major groups might consist of Blastocystis isolates from both humans and other animal hosts, confirming the low host specificity of Blastocystis. Our results also strongly suggest the existence of numerous zoonotic isolates with frequent animal-to-human and human-to-animal transmissions and of a large potential reservoir in animals for infections in humans.en
dc.description.sponsorshipThis work was supported by the Institut Pasteur de Lille, the Institut National de la Sante´ et de la Recherche Me´dicale, the Centre National de la Recherche Scientifique, the Institut National de la Recherche Agronomique, the Ecole Ve´te´rinaire de Lyon, and the “Programme Trans-Zoonoses 2002–2004” (INRA—De´partement de Sante´ Animale).en
dc.format.extent206776 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen
dc.publisherAmerican Society for Microbiologyen
dc.relation.urihttps://doi.org/10.1128/JCM.43.1.348-355.2005
dc.subjectAnimal-to-human transmissionsen
dc.subjectHuman-to-animal transmissionsen
dc.titleMolecular phylogenies of Blastocystis isolates from different hosts : implications for genetic diversity, identification of species, and zoonosisen
dc.typeArticleen
dc.identifier.doi10.1128/JCM.43.1.348-355.2005


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