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    Molecular phylogenies of Blastocystis isolates from different hosts : implications for genetic diversity, identification of species, and zoonosis

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    Noel Molecular.pdf (201.9Kb)
    Date
    2005-01
    Author
    Noel, Christophe  Concept link
    Dufernez, Fabienne  Concept link
    Gerbod, Delphine  Concept link
    Edgcomb, Virginia P.  Concept link
    Delgado-Viscogliosi, Pilar  Concept link
    Ho, Lip-Chuen  Concept link
    Singh, Mulkit  Concept link
    Wintjens, Rene  Concept link
    Sogin, Mitchell L.  Concept link
    Capron, Monique  Concept link
    Pierce, Raymond  Concept link
    Zenner, Lionel  Concept link
    Viscogliosi, Eric  Concept link
    Metadata
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    Citable URI
    https://hdl.handle.net/1912/182
    As published
    https://doi.org/10.1128/JCM.43.1.348-355.2005
    DOI
    10.1128/JCM.43.1.348-355.2005
    Keyword
     Animal-to-human transmissions; Human-to-animal transmissions 
    Abstract
    Small-subunit (SSU) rRNA gene sequences were obtained by PCR from 12 Blastocystis isolates from humans, rats, and reptiles for which elongation factor 1{alpha} (EF-1{alpha}) gene sequences are already available. These new sequences were analyzed by the Bayesian method in a broad phylogeny including, for the first time, all Blastocystis sequences available in the databases. Phylogenetic trees identified seven well-resolved groups plus several discrete lineages that could represent newly defined clades. Comparative analysis of SSU rRNA- and EF-1{alpha}-based trees obtained by maximum-likelihood methods from a restricted sampling (13 isolates) revealed overall agreement between the two phylogenies. In spite of their morphological similarity, sequence divergence among Blastocystis isolates reflected considerable genetic diversity that could be correlated with the existence of potentially ≥12 different species within the genus. Based on this analysis and previous PCR-based genotype classification data, six of these major groups might consist of Blastocystis isolates from both humans and other animal hosts, confirming the low host specificity of Blastocystis. Our results also strongly suggest the existence of numerous zoonotic isolates with frequent animal-to-human and human-to-animal transmissions and of a large potential reservoir in animals for infections in humans.
    Description
    Author Posting. © American Society for Microbiology, 2005. This article is posted here by permission of American Society for Microbiology for personal use, not for redistribution. The definitive version was published in Journal of Clinical Microbiology 43 (2005): 348-355, doi:10.1128/JCM.43.1.348-355.2005.
    Collections
    • Biology
    • Josephine Bay Paul Center in Comparative Molecular Biology and Evolution
    Suggested Citation
    Article: Noel, Christophe, Dufernez, Fabienne, Gerbod, Delphine, Edgcomb, Virginia P., Delgado-Viscogliosi, Pilar, Ho, Lip-Chuen, Singh, Mulkit, Wintjens, Rene, Sogin, Mitchell L., Capron, Monique, Pierce, Raymond, Zenner, Lionel, Viscogliosi, Eric, "Molecular phylogenies of Blastocystis isolates from different hosts : implications for genetic diversity, identification of species, and zoonosis", Journal of Clinical Microbiology 43 (2005): 348-355, DOI:10.1128/JCM.43.1.348-355.2005, https://hdl.handle.net/1912/182
     

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