Signature lipids and stable carbon isotope analyses of Octopus Spring hyperthermophilic communities compared with those of aquificales representatives
Jahnke, Linda L.
Hope, Janet M.
Hayes, John M.
Des Marais, David J.
Cady, Sherry L.
Summons, Roger E.
MetadataShow full item record
The molecular and isotopic compositions of lipid biomarkers of cultured Aquificales genera have been used to study the community and trophic structure of the hyperthermophilic pink streamers and vent biofilm from Octopus Spring. Thermocrinis ruber, Thermocrinis sp. strain HI 11/12, Hydrogenobacter thermophilus TK-6, Aquifex pyrophilus, and Aquifex aeolicus all contained glycerol-ether phospholipids as well as acyl glycerides. The n-C20:1 and cy-C21 fatty acids dominated all of the Aquificales, while the alkyl glycerol ethers were mainly C18:0. These Aquificales biomarkers were major constituents of the lipid extracts of two Octopus Spring samples, a biofilm associated with the siliceous vent walls, and the well-known pink streamer community (PSC). Both the biofilm and the PSC contained mono- and dialkyl glycerol ethers in which C18 and C20 alkyl groups were prevalent. Phospholipid fatty acids included both the Aquificales n-C20:1 and cy-C21, plus a series of iso-branched fatty acids (i-C15:0 to i-C21:0), indicating an additional bacterial component. Biomass and lipids from the PSC were depleted in 13C relative to source water CO2 by 10.9 and 17.2per thousand , respectively. The C20-21 fatty acids of the PSC were less depleted than the iso-branched fatty acids, 18.4 and 22.6per thousand , respectively. The biomass of T. ruber grown on CO2 was depleted in 13C by only 3.3per thousand relative to C source. In contrast, biomass was depleted by 19.7per thousand when formate was the C source. Independent of carbon source, T. ruber lipids were heavier than biomass (+1.3per thousand ). The depletion in the C20-21 fatty acids from the PSC indicates that Thermocrinis biomass must be similarly depleted and too light to be explained by growth on CO2. Accordingly, Thermocrinis in the PSC is likely to have utilized formate, presumably generated in the spring source region.
Author Posting. © American Society for Microbiology, 2001. This article is posted here by permission of American Society for Microbiology for personal use, not for redistribution. The definitive version was published in Applied and Environmental Microbiology 67 (2001): 5179-5189, doi:10.1128/AEM.67.11.5179-5189.2001.
Suggested CitationArticle: Jahnke, Linda L., Eder, Wolfgang, Huber, Robert, Hope, Janet M., Hinrichs, Kai-Uwe, Hayes, John M., Des Marais, David J., Cady, Sherry L., Summons, Roger E., "Signature lipids and stable carbon isotope analyses of Octopus Spring hyperthermophilic communities compared with those of aquificales representatives", Applied and Environmental Microbiology 67 (2001): 5179-5189, DOI:10.1128/AEM.67.11.5179-5189.2001, https://hdl.handle.net/1912/180
Showing items related by title, author, creator and subject.
An autonomous, in situ light-dark bottle device for determining community respiration and net community production Collins, James R.; Fucile, Paul D.; McDonald, Glenn; Ossolinski, Justin E.; Keil, Richard G.; Valdes, James R.; Doney, Scott C.; Van Mooy, Benjamin A. S. (2018-03)We describe a new, autonomous, incubation-based instrument that is deployed in situ to determine rates of gross community respiration and net community production in marine and aquatic ecosystems. During deployments at ...
Ciliate diversity, community structure, and novel taxa in lakes of the McMurdo Dry Valleys, Antarctica Xu, Yuan; Vick-Majors, Trista; Morgan-Kiss, Rachael; Priscu, John C.; Amaral-Zettler, Linda A. (Marine Biological Laboratory, 2014-10-01)We report an in-depth survey of next-generation DNA sequencing of ciliate diversity and community structure in two permanently ice-covered McMurdo Dry Valley lakes during the austral summer and autumn (November 2007 and ...
CHANS : modeling the dynamics of HABs, human communities, and policy choices along the Florida Gulf Coast Hoagland, Porter; Kirkpatrick, Barbara; Kirkpatrick, Gary; Hitchcock, Gary; Ullmann, Steven G.; Reich, Andrew; Fleming, Lora E.; Jin, Di; Beet, Andrew R.; Li, Cathy; Garrison, Bruce; Lovko, Vince; Kohler, Kate; Rudge, Katrin (2015-11-19)Coupled human-nature systems (CHANS) involve dynamic interactions between humans and nature, often influenced by and affecting the distinct dynamic characteristics of each component. We present an overview of an ongoing ...