Characterization of methyl sugars, 3-deoxysugars and methyl deoxysugars in marine high molecular weight dissolved organic matter

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2007-01-16Author
Panagiotopoulos, Christos
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Repeta, Daniel J.
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Johnson, Carl G.
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https://hdl.handle.net/1912/1794As published
https://doi.org/10.1016/j.orggeochem.2007.02.005Abstract
Nuclear magnetic resonance spectroscopy of marine high molecular weight
dissolved organic matter (HMWDOM) in surface waters show that >50% of the carbon is
a compositionally well-defined family of acylated-polysaccharides that are conserved
across ocean basins. However, acid hydrolysis of HMWDOM followed by
chromatographic analyses recover only 10-20% of the carbon as neutral, amino, and
acidic sugars. Most carbohydrate in HMWDOM therefore remains uncharacterized.
Here we use acid hydrolysis followed by Ag+ and Pb2+ cation exchange chromatography
to separate HMWDOM hydrolysis products for characterization by 1-D and 2-D NMR
spectroscopy. In addition to neutral sugars identified in past studies, we find 3-Omethylglucose,
3-O-methylrhamnose, 2-O-methylrhamnose and 2-O-methylfucose. We
also find 3-deoxysugars to be present, although their complete structures could not be
determined. Methyl sugars are widely distributed in plant and bacterial structural
carbohydrates, such as cell wall polysaccharides, and their presence in HMWDOM
suggests that structural carbohydrates may contribute to DOM in surface seawater. We
find most HMWDOM carbohydrate is not depolymerized by acid hydrolysis, and that the
nonhydrolyzable component includes 6-deoxysugars.
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Author Posting. © Elsevier B.V., 2007. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Organic Geochemistry 38 (2007): 884-896, doi:10.1016/j.orggeochem.2007.02.005.
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Preprint: Panagiotopoulos, Christos, Repeta, Daniel J., Johnson, Carl G., "Characterization of methyl sugars, 3-deoxysugars and methyl deoxysugars in marine high molecular weight dissolved organic matter", 2007-01-16, https://doi.org/10.1016/j.orggeochem.2007.02.005, https://hdl.handle.net/1912/1794Related items
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