Sequence motif within trypanosome precursor tRNAs influences abundance and mitochondrial localization
Sherrer, R. Lynn
Yermovsky-Kammerer, Audra E.
Hajduk, Stephen L.
MetadataShow full item record
Trypanosoma brucei lacks mitochondrial genes encoding tRNAs and must import nuclearly encoded tRNAs from the cytosol. The mechanism and specificity of this process remain unclear. We have identified a unique sequence motif, YGG(C/A)RRC, upstream of the genes encoding mitochondrially localized tRNAs in T. brucei. Both in vitro import studies and in vivo transfection studies indicate that deletion of the YGG(C/A)RRC sequence alters mitochondrial localization of tRNALeu, and in vivo studies also show a decrease in the cellular abundance of tRNALeu. These studies provide direct evidence for cis-acting RNA motifs within precursor tRNAs that facilitate the selection of tRNAs for mitochondrial import in trypanosomes. Furthermore, we found that mutations to the YGG(C/A)RRC sequence also altered the intracellular distribution of other endogenous tRNAs, suggesting a general role for this sequence in tRNA trafficking in trypanosomes.
Author Posting. © American Society for Microbiology, 2003. This article is posted here by permission of American Society for Microbiology for personal use, not for redistribution. The definitive version was published in Molecular and Cellular Biology 23 (2003): 9061-9072, doi:10.1128/MCB.23.24.9061-9072.2003.
Showing items related by title, author, creator and subject.
Mitochondrial genomes of Clymenella torquata (Maldanidae) and Riftia pachyptila (Siboglinidae) : evidence for conserved gene order in Annelida Jennings, Robert M.; Halanych, Kenneth M. (2004-10-04)Mitochondrial genomes are useful tools for inferring evolutionary history. However, many taxa are poorly represented by available data. Thus, to further understand the phylogenetic potential of complete mitochondrial ...
Jennings, Robert M. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 2005-09)The overarching goal of this thesis was to investigate marine benthic invertebrate phylogenetics and population genetics, focused on the phylum Annelida. Recent expansions of molecular methods and the increasing diversity ...
Mitochondrial DNA sequence variation in North Atlantic long-finned pilot whales, Globicephala melas Siemann, Liese A. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 1994-05)I sequenced mitochondrial DNA (mtDNA) from 59 long-finned pilot whales (Globicephala melas) that stranded on the coasts of Cape Cod, Newfoundland, Nova Scotia, Scotland, and England or were caught by commercial fisheries ...