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    Importance of passive diffusion in the uptake of polychlorinated biphenyls by phagotrophic protozoa

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    Kujawinski Importance.pdf (118.0Kb)
    Date
    2000-05
    Author
    Kujawinski, Elizabeth B.  Concept link
    Farrington, John W.  Concept link
    Moffett, James W.  Concept link
    Metadata
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    Citable URI
    https://hdl.handle.net/1912/169
    As published
    https://doi.org/10.1128/AEM.66.5.1987-1993.2000
    DOI
    10.1128/AEM.66.5.1987-1993.2000
    Keyword
     Polychlorinated biphenyls; Grazing and nongrazing protozoa; Uptake and loss processes 
    Abstract
    Unicellular protozoan grazers represent a size class of organisms where a transition in the mechanism of chlorobiphenyl (CB) introduction, from diffusion through surface membranes to ingestion of contaminated prey, could occur. This study compares the relative importance of these two processes in the overall uptake of polychlorinated biphenyls by protists. Uptake rates and steady-state concentrations were compared in laboratory cultures of grazing and nongrazing protozoa. These experiments were conducted with a 10-µm marine scuticociliate (Uronema sp.), bacterial prey (Halomonas halodurans), and a suite of 21 CB congeners spanning a range of aqueous solubilities. The dominant pathway of CB uptake by both grazing and nongrazing protozoa was diffusion. Organic-carbon-normalized CB concentrations (in the protozoan cell) were equivalent in grazing and nongrazing protozoa for all congeners studied. Rate constants for uptake into and loss from the protozoan cell were independently determined by using [3,3',4,4'-14C]tetrachlorobiphenyl (IUPAC no. 77), 0.38 ± 0.03 min-1 and (1.1 ± 0.1) × 10-5 (g of organic carbon)-1 min-1, respectively. Magnitudes of the uptake and loss processes were calculated and compared by using a numerical model. The model result was consistent with data from the bioaccumulation experiment and supported the hypothesis that diffusive uptake is faster than ingestive uptake in phagotrophic unicellular protozoa.
    Description
    Author Posting. © American Society for Microbiology, 2000. This article is posted here by permission of American Society for Microbiology for personal use, not for redistribution. The definitive version was published in Applied and Environmental Microbiology 66 (2000): 1987-1993, doi:10.1128/AEM.66.5.1987-1993.2000.
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    • Marine Chemistry and Geochemistry (MC&G)
    Suggested Citation
    Applied and Environmental Microbiology 66 (2000): 1987-1993
     

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