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dc.contributor.authorSchaus, Thomas E.  Concept link
dc.contributor.authorTaylor, Edwin W.  Concept link
dc.contributor.authorBorisy, Gary G.  Concept link
dc.date.accessioned2007-05-18T18:02:42Z
dc.date.available2007-05-18T18:02:42Z
dc.date.issued2006-11-16
dc.identifier.urihttps://hdl.handle.net/1912/1642
dc.descriptionAuthor Posting. © The Author(s), 2006. This is the author's version of the work. It is posted here by permission of National Academy of Sciences of the USA for personal use, not for redistribution. The definitive version was published in Proceedings of the National Academy of Sciences 104 (2007): 7086-7091, doi:10.1073/pnas.0701943104.en
dc.description.abstractThe dendritic-nucleation/array-treadmilling model provides a conceptual framework for the generation of the actin network driving motile cells. We have incorporated it into a 2-D, stochastic computer model to study lamellipodia via the self-organization of filament orientation patterns. Essential dendritic-nucleation sub-models were incorporated, including discretized actin monomer diffusion, Monte-Carlo filament kinetics, and flexible filament and plasma membrane mechanics. Model parameters were estimated from the literature and simulation, providing values for the extent of the leading edge branching/capping-protective zone (5.4 nm) and the auto-catalytic branch rate (0.43 /s). For a given set of parameters the system evolved to a steady state filament count and velocity, at which total branching and capping rates were equal only for specific orientations; net capping eliminated others. The standard parameter set evoked a sharp preference for the ±35 deg. filaments seen in lamellipodial electron micrographs, requiring ~ 12 generations of successive branching to adapt to a 15 deg. change in protrusion direction. This pattern was robust with respect to membrane surface and bending energies and to actin concentrations, but required protection from capping at the leading edge and branching angles greater than 60 deg. A +70/0/-70 deg. pattern was formed with flexible filaments ~ 100 nm or longer and with velocities less than ~ 20% of free polymerization rates.en
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen
dc.relation.urihttps://doi.org/10.1073/pnas.0701943104
dc.subjectLamellipodiumen
dc.subjectCytoskeletonen
dc.subjectPlasma membraneen
dc.titleSelf-organization of actin filament orientation in the dendritic-nucleation/array-treadmilling modelen
dc.typePreprinten


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