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    Protein phosphatase 2A plays a crucial role in Giardia lamblia differentiation

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    Gillin et al.pdf (1.602Mb)
    Date
    2006-12-06
    Author
    Lauwaet, Tineke  Concept link
    Davids, Barbara J.  Concept link
    Torres-Escobar, Ascencion  Concept link
    Birkeland, Shanda R.  Concept link
    Cipriano, Michael J.  Concept link
    Preheim, Sarah P.  Concept link
    Palm, Daniel  Concept link
    Svard, Staffan G.  Concept link
    McArthur, Andrew G.  Concept link
    Gillin, Frances D.  Concept link
    Metadata
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    Citable URI
    https://hdl.handle.net/1912/1529
    As published
    https://doi.org/10.1016/j.molbiopara.2006.12.001
    Keyword
     Giardia; Encystation; Excystation; PP2A; Cytoskeleton; Differentiation 
    Abstract
    The ability of Giardia lamblia to undergo two distinct differentiations in response to physiologic stimuli is central to its pathogenesis. The giardial cytoskeleton changes drastically during encystation and excystation. However, the signal transduction pathways mediating these transformations are poorly understood. We tested the hypothesis that PP2A, a highly conserved serine/threonine protein phosphatase, might be important in giardial differentiation. We found that in vegetatively growing trophozoites, gPP2A-C protein localizes to basal bodies/centrosomes, and to cytoskeletal structures unique to Giardia: the ventral disk, and the dense rods of the anterior, posterior-lateral, and caudal flagella. During encystation, gPP2A-C protein disappears from only the anterior flagellar dense rods. During excystation, gPP2A-C localizes to the cyst wall in excysting cysts but is not found in the wall of cysts with emerging excyzoites. Transcriptome and immunoblot analyses indicated that gPP2A-C mRNA and protein are upregulated in mature cysts and during the early stage of excystation that models passage through the host stomach. Stable expression of gPP2A-C antisense RNA did not affect vegetative growth, but strongly inhibited the formation of encystation secretory vesicles (ESV) and water-resistant cysts. Moreover, the few cysts that formed were highly defective in excystation. Thus, gPP2A-C localizes to universal cytoskeletal structures and to structures unique to Giardia. It is also important for encystation and excystation, crucial giardial transformations that entail entry into and exit from dormancy.
    Description
    Author Posting. © The Authors, 2006. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Molecular and Biochemical Parasitology 152 (2007): 80-89, doi:10.1016/j.molbiopara.2006.12.001.
    Collections
    • Josephine Bay Paul Center in Comparative Molecular Biology and Evolution
    Suggested Citation
    Preprint: Lauwaet, Tineke, Davids, Barbara J., Torres-Escobar, Ascencion, Birkeland, Shanda R., Cipriano, Michael J., Preheim, Sarah P., Palm, Daniel, Svard, Staffan G., McArthur, Andrew G., Gillin, Frances D., "Protein phosphatase 2A plays a crucial role in Giardia lamblia differentiation", 2006-12-06, https://doi.org/10.1016/j.molbiopara.2006.12.001, https://hdl.handle.net/1912/1529
     

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