Physiological proteomics of the uncultured endosymbiont of Riftia pachyptila


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dc.contributor.author Markert, Stephanie
dc.contributor.author Arndt, Cordelia
dc.contributor.author Felbeck, Horst
dc.contributor.author Becher, Dorte
dc.contributor.author Sievert, Stefan M.
dc.contributor.author Hugler, Michael
dc.contributor.author Albrecht, Dirk
dc.contributor.author Robidart, Julie
dc.contributor.author Bench, Shellie
dc.contributor.author Feldman, Robert A.
dc.contributor.author Hecker, Michael
dc.contributor.author Schweder, Thomas
dc.date.accessioned 2007-02-28T14:25:14Z
dc.date.available 2007-02-28T14:25:14Z
dc.date.issued 2006-11-14
dc.identifier.uri http://hdl.handle.net/1912/1514
dc.description Author Posting. © The Authors, 2006. This is the author's version of the work. It is posted here by permission of AAAS for personal use, not for redistribution. The definitive version was published in Science 315 (2007): 247-250, doi:10.1126/science.1132913. en
dc.description.abstract The bacterial endosymbiont of the deep-sea tube worm Riftia pachyptila has never been successfully cultivated outside its host. In the absence of cultivation data we have taken a proteomic approach based on the metagenome sequence to study the metabolism of this peculiar microorganism in detail. As one result, we found that three major sulfide oxidation proteins constitute ~12% of the total cytosolic proteome, highlighting the essential role of these enzymes for the symbiont’s energy metabolism. Unexpectedly, the symbiont uses the reductive tricarboxylic acid (TCA) cycle in addition to the previously identified Calvin cycle for CO2 fixation. en
dc.description.sponsorship This work was supported by the DFG, grant Schw595/3-1. Other funding sources were: NSF (OCE 04-52333) and NASA Astrobiology Institute (NNA04CC04A) for SMS, MH: postdoctoral scholarship from WHOI, HF: Academic Senate (RF811S and RE518S). en
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dc.language.iso en_US en
dc.relation.uri http://dx.doi.org/10.1126/science.1132913
dc.title Physiological proteomics of the uncultured endosymbiont of Riftia pachyptila en
dc.title.alternative Approaching the uncultured endosymbiont of Riftia pachyptila by physiological proteomics en
dc.type Preprint en

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