Physiological proteomics of the uncultured endosymbiont of Riftia pachyptila

View/ Open
Date
2006-11-14Author
Markert, Stephanie
Concept link
Arndt, Cordelia
Concept link
Felbeck, Horst
Concept link
Becher, Dorte
Concept link
Sievert, Stefan M.
Concept link
Hugler, Michael
Concept link
Albrecht, Dirk
Concept link
Robidart, Julie
Concept link
Bench, Shellie
Concept link
Feldman, Robert A.
Concept link
Hecker, Michael
Concept link
Schweder, Thomas
Concept link
Metadata
Show full item recordCitable URI
https://hdl.handle.net/1912/1514As published
https://doi.org/10.1126/science.1132913Abstract
The bacterial endosymbiont of the deep-sea tube worm Riftia pachyptila has
never been successfully cultivated outside its host. In the absence of cultivation data we
have taken a proteomic approach based on the metagenome sequence to study the
metabolism of this peculiar microorganism in detail. As one result, we found that three
major sulfide oxidation proteins constitute ~12% of the total cytosolic proteome,
highlighting the essential role of these enzymes for the symbiont’s energy metabolism.
Unexpectedly, the symbiont uses the reductive tricarboxylic acid (TCA) cycle in addition
to the previously identified Calvin cycle for CO2 fixation.
Description
Author Posting. © The Authors, 2006. This is the author's version of the work. It is posted here by permission of AAAS for personal use, not for redistribution. The definitive version was published in Science 315 (2007): 247-250, doi:10.1126/science.1132913.
Collections
Suggested Citation
Preprint: Markert, Stephanie, Arndt, Cordelia, Felbeck, Horst, Becher, Dorte, Sievert, Stefan M., Hugler, Michael, Albrecht, Dirk, Robidart, Julie, Bench, Shellie, Feldman, Robert A., Hecker, Michael, Schweder, Thomas, "Physiological proteomics of the uncultured endosymbiont of Riftia pachyptila", 2006-11-14, https://doi.org/10.1126/science.1132913, https://hdl.handle.net/1912/1514Related items
Showing items related by title, author, creator and subject.
-
Deadly diving? Physiological and behavioural management of decompression stress in diving mammals
Hooker, Sascha K.; Fahlman, Andreas; Moore, Michael J.; Aguilar De Soto, Natacha; Bernaldo de Quiros, Yara; Brubakk, A. O.; Costa, Daniel P.; Costidis, Alexander M.; Dennison, Sophie; Falke, K. J.; Fernandez, Antonio; Ferrigno, Massimo; Fitz-Clarke, J. R.; Garner, Michael M.; Houser, Dorian S.; Jepson, Paul D.; Ketten, Darlene R.; Kvadsheim, P. H.; Madsen, Peter T.; Pollock, N. W.; Rotstein, David S.; Rowles, Teresa K.; Simmons, S. E.; Van Bonn, William; Weathersby, P. K.; Weise, Michael; Williams, Terrie M.; Tyack, Peter L. (Royal Society, 2011-12-21)Decompression sickness (DCS; ‘the bends’) is a disease associated with gas uptake at pressure. The basic pathology and cause are relatively well known to human divers. Breath-hold diving marine mammals were thought to be ... -
Live-cell imaging RNAi screen identifies PP2A–B55α and importin-β1 as key mitotic exit regulators in human cells
Schmitz, Michael H. A.; Held, Michael; Janssens, Veerle; Hutchins, James R. A.; Hudecz, Otto; Ivanova, Elitsa; Goris, Jozef; Trinkle-Mulcahy, Laura; Lamond, Angus I.; Poser, Ina; Hyman, Anthony A.; Mechtler, Karl; Peters, Jan-Michael; Gerlich, Daniel W. (2010-07)When vertebrate cells exit mitosis, they reorganize various cellular structures to build functional interphase cells1. This depends on Cdk1 inactivation and subsequent dephosphorylation of its substrates2-4. Members of ... -
Quantity, composition, and source of sediment collected in sediment traps along the fringing coral reef off Molokai, Hawaii
Bothner, Michael H.; Reynolds, Richard L.; Casso, Michael A.; Storlazzi, Curt D.; Field, Michael E. (Elsevier B.V., 2006-03-20)Sediment traps were used to evaluate the frequency, cause, and relative intensity of sediment mobility/resuspension along the fringing coral reef off southern Molokai (February 2000–May 2002). Two storms with high rainfall, ...