Physiological proteomics of the uncultured endosymbiont of Riftia pachyptila
Citable URI
http://hdl.handle.net/1912/1514As published
http://dx.doi.org/10.1126/science.1132913Abstract
The bacterial endosymbiont of the deep-sea tube worm Riftia pachyptila has
never been successfully cultivated outside its host. In the absence of cultivation data we
have taken a proteomic approach based on the metagenome sequence to study the
metabolism of this peculiar microorganism in detail. As one result, we found that three
major sulfide oxidation proteins constitute ~12% of the total cytosolic proteome,
highlighting the essential role of these enzymes for the symbiont’s energy metabolism.
Unexpectedly, the symbiont uses the reductive tricarboxylic acid (TCA) cycle in addition
to the previously identified Calvin cycle for CO2 fixation.
Description
Author Posting. © The Authors, 2006. This is the author's version of the work. It is posted here by permission of AAAS for personal use, not for redistribution. The definitive version was published in Science 315 (2007): 247-250, doi:10.1126/science.1132913.
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