Physiological proteomics of the uncultured endosymbiont of Riftia pachyptila

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Date
2006-11-14
Author
Markert, Stephanie 
Arndt, Cordelia 
Felbeck, Horst 
Becher, Dorte 
Sievert, Stefan M. 
Hugler, Michael 
Albrecht, Dirk 
Robidart, Julie 
Bench, Shellie 
Feldman, Robert A. 
Hecker, Michael 
Schweder, Thomas 
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https://hdl.handle.net/1912/1514
As published
https://doi.org/10.1126/science.1132913
Abstract
The bacterial endosymbiont of the deep-sea tube worm Riftia pachyptila has never been successfully cultivated outside its host. In the absence of cultivation data we have taken a proteomic approach based on the metagenome sequence to study the metabolism of this peculiar microorganism in detail. As one result, we found that three major sulfide oxidation proteins constitute ~12% of the total cytosolic proteome, highlighting the essential role of these enzymes for the symbiont’s energy metabolism. Unexpectedly, the symbiont uses the reductive tricarboxylic acid (TCA) cycle in addition to the previously identified Calvin cycle for CO2 fixation.
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Author Posting. © The Authors, 2006. This is the author's version of the work. It is posted here by permission of AAAS for personal use, not for redistribution. The definitive version was published in Science 315 (2007): 247-250, doi:10.1126/science.1132913.
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Preprint: Markert, Stephanie, Arndt, Cordelia, Felbeck, Horst, Becher, Dorte, Sievert, Stefan M., Hugler, Michael, Albrecht, Dirk, Robidart, Julie, Bench, Shellie, Feldman, Robert A., Hecker, Michael, Schweder, Thomas, "Physiological proteomics of the uncultured endosymbiont of Riftia pachyptila", 2006-11-14, https://doi.org/10.1126/science.1132913, https://hdl.handle.net/1912/1514