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217 000-year-old DNA sequences of green sulfur bacteria in Mediterranean sapropels and their implications for the reconstruction of the paleoenvironment

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dc.contributor.author Coolen, Marco J. L.
dc.contributor.author Overmann, Jorg
dc.date.accessioned 2007-02-15T15:15:34Z
dc.date.available 2007-02-15T15:15:34Z
dc.date.issued 2006-08-08
dc.identifier.uri http://hdl.handle.net/1912/1506
dc.description Author Posting. © The Authors, 2006. This is the author's version of the work. It is posted here by permission of Society for Applied Microbiology and Blackwell for personal use, not for redistribution. The definitive version was published in Environmental Microbiology 9 (2007): 238–249, doi:10.1111/j.1462-2920.2006.01134.x. en
dc.description.abstract Deep-sea sediments of the eastern Mediterranean harbor a series of dark, organic carbon-rich layers, so-called sapropels. Within these layers, the carotenoid isorenieratene was detected. Since it is specific for the obligately anaerobic phototrophic green sulfur bacteria, the presence of isorenieratene may suggest that extended water column anoxia occurred in the ancient Mediterranean Sea during periods of sapropel formation. Only three carotenoids (isorenieratene, β-isorenieratene and chlorobactene) are typical for green sulfur bacteria and thus do not permit to differentiate between the ~80 known phylotypes. In order to reconstruct the paleoecological conditions in more detail, we searched for fossil 16S rRNA gene sequences of green sulfur bacteria employing ancient DNA methodology. 540 bp-long fossil sequences could indeed be amplified from up to 217,000-year-old sapropels. In addition, such sequences were also recovered from carbon-lean intermediate sediment layers deposited during times of an entirely oxic water column. Unexpectedly, however, all the recovered 16S rRNA gene sequences grouped with freshwater or brackish, rather than truly marine, types of green sulfur bacteria. It is therefore feasible that the molecular remains of green sulfur bacteria originated from populations which thrived in adjacent freshwater or estuarine coastal environments rather than from an indigenous pelagic population. en
dc.description.sponsorship This work was funded by the Deutsche Forschungsgemeinschaft (grants Ov 20/3-2 and Ov 20/8-1 to 8-3). en
dc.format.extent 1959956 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US en
dc.relation.uri http://dx.doi.org/10.1111/j.1462-2920.2006.01134.x
dc.subject Ancient DNA en
dc.subject Oceanic anoxic events en
dc.subject Chlorobiaceae en
dc.subject Fossil DNA en
dc.subject Green sulfur bacteria en
dc.subject Sapropels en
dc.title 217 000-year-old DNA sequences of green sulfur bacteria in Mediterranean sapropels and their implications for the reconstruction of the paleoenvironment en
dc.type Preprint en


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