Diversity and distribution of subseafloor Thermococcales populations in diffuse hydrothermal vents at an active deep-sea volcano in the northeast Pacific Ocean
MetadataShow full item record
KeywordSubseafloor; Hydrothermal vent; Intergenic Transcribed Spacer (ITS) region; Geochemistry; Diffuse flow vent
The presence, diversity, and distribution of a key group of subseafloor archaea, the Thermococcales, was examined in multiple diffuse flow hydrothermal vents at Axial Seamount, an active deep-sea volcano located in the northeast Pacific Ocean. A polymerase chain reaction (PCR) approach was used to determine if this group of subseafloor indicator organisms showed any phylogenetic distribution that may indicate distinct subseafloor communities at vents with different physical and chemical characteristics. Targeted primers for the Thermococcales 16S rRNA (small subunit ribosomal RNA) gene and intergenic transcribed spacer (ITS) region were designed and applied to organisms filtered in-situ directly from a variety of diffuse flow vents. Thermococcales were amplified from 9 of 11 samples examined, and it was determined that the ITS region is a better phylogenetic marker than the 16S rRNA in defining consistent groups of closely related sequences. Results show a relationship between environmental clone distribution and source vent chemistry. The most highly diluted vents with elevated iron and alkalinity contained a distinct group of Thermococcales as defined by the ITS region, suggesting separate subseafloor Thermococcales populations at diffuse vents within the Axial caldera.
Author Posting. © American Geophysical Union, 2006. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research 111 (2006): G04016, doi:10.1029/2005JG000097.
Suggested CitationArticle: Huber, Julie A., Butterfield, David A., Baross, John A., "Diversity and distribution of subseafloor Thermococcales populations in diffuse hydrothermal vents at an active deep-sea volcano in the northeast Pacific Ocean", Journal of Geophysical Research 111 (2006): G04016, DOI:10.1029/2005JG000097, https://hdl.handle.net/1912/1435
Showing items related by title, author, creator and subject.
Rare earth element abundances in hydrothermal fluids from the Manus Basin, Papua New Guinea : indicators of sub-seafloor hydrothermal processes in back-arc basins Craddock, Paul R.; Bach, Wolfgang; Seewald, Jeffrey S.; Rouxel, Olivier J.; Reeves, Eoghan P.; Tivey, Margaret K. (2010-05-02)Rare earth element (REE) concentrations are reported for a large suite of seafloor vent fluids from four hydrothermal systems in the Manus back–arc basin (Vienna Woods, PACMANUS, DESMOS and SuSu Knolls vent areas). Sampled ...
Genomic variation in microbial populations inhabiting the marine subseafloor at deep-sea hydrothermal vents Anderson, Rika E.; Reveillaud, Julie; Reddington, Emily; Delmont, Tom O.; Eren, A. Murat; McDermott, Jill M.; Seewald, Jeffrey S.; Huber, Julie A. (Nature Publishing Group, 2017-10-24)Little is known about evolutionary drivers of microbial populations in the warm subseafloor of deep-sea hydrothermal vents. Here we reconstruct 73 metagenome-assembled genomes (MAGs) from two geochemically distinct vent ...
Crustal magnetization and the subseafloor structure of the ASHES vent field, Axial Seamount, Juan de Fuca Ridge : implications for the investigation of hydrothermal sites Tontini, F. Caratori; Crone, Timothy J.; de Ronde, Cornel E. J.; Fornari, Daniel J.; Kinsey, James C.; Mittelstaedt, Eric; Tivey, Maurice A. (John Wiley & Sons, 2016-06-24)High-resolution geophysical data have been collected using the Autonomous Underwater Vehicle (AUV) Sentry over the ASHES (Axial Seamount Hydrothermal Emission Study) high-temperature (~348°C) vent field at Axial Seamount, ...