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    A New invertebrate member of the p53 gene family is developmentally expressed and responds to polychlorinated biphenyls

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    Jessen Eller et al.pdf (1.572Mb)
    Date
    2002-03-07
    Author
    Jessen-Eller, Kathryn  Concept link
    Kreiling, Jill A.  Concept link
    Begley, Gail S.  Concept link
    Steele, Marjorie E.  Concept link
    Walker, Charles W.  Concept link
    Stephens, Raymond E.  Concept link
    Reinisch, Carol L.  Concept link
    Metadata
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    Citable URI
    https://hdl.handle.net/1912/129
    As published
    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1240800/
    Keyword
     Neurotoxicology; p63; p73; PCBs; Surf clam; Spisula solidissima 
    Abstract
    The cell-cycle checkpoint protein p53 both directs terminal differentiation and protects embryos from DNA damage. To study invertebrate p53 during early development, we identified three differentially expressed p53 family members (p53, p97, p120) in the surf clam, Spisula solidissima. In these mollusks, p53 and p97 occur in both embryonic and adult tissue, whereas p120 is exclusively embryonic. We sequenced, cloned, and characterized p120 cDNA. The predicted protein, p120, resembles p53 across all evolutionarily conserved regions and contains a C-terminal extension with a sterile alpha motif (SAM) as in p63 and p73. These vertebrate forms of p53 are required for normal inflammatory, epithelial, and neuronal development. Unlike clam p53 and p97, p120 mRNA and protein levels are temporally expressed in embryos, with mRNA levels decreasing with increasing p120 protein (R2 = 0.97). Highest surf clam p120 mRNA levels coincide with the onset of neuronal growth. In earlier work we have shown that neuronal development is altered by exposure to polychlorinated biphenyls (PCBs), a neurotoxic environmental contaminant. In this study we show that PCBs differentially affect expression of the three surf clam p53 family members. p120 mRNA and protein are reduced the most and earliest in development, p97 protein shows a smaller and later reduction, and p53 protein levels do not change. For the first time we report that unlike p53 and p97, p120 is specifically embryonic and expressed in a time-dependent manner. Furthermore, p120 responds to PCBs by 48 hr when PCB-induced suppression of the serotonergic nervous system occurs.
    Description
    Author Posting. EHP is a publication of the United States government. Publication of EHP lies in the public domain and is therefore without copyright. The definitive version was published in Environmental Health Perspectives Environmental Health Perspectives 110 (2002): 377-385.
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    • Laboratory of Aquatic Biomedicine
    Suggested Citation
    Environmental Health Perspectives 110 (2002): 377-385
     
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