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dc.contributor.authorPielak, Rafal M.
dc.contributor.authorHawkins, Christopher
dc.contributor.authorPyie, Aung
dc.contributor.authorBautista, Jennifer
dc.contributor.authorLee, Kyeng-Gea
dc.contributor.authorCohen, William D.
dc.date.accessioned2006-10-04T20:21:45Z
dc.date.available2006-10-04T20:21:45Z
dc.date.issued2005-08
dc.identifier.citationBiological Bulletin 209 (2005): 21-30en
dc.identifier.urihttp://hdl.handle.net/1912/1260
dc.descriptionAuthor Posting. © Marine Biological Laboratory, 2005. This article is posted here by permission of Marine Biological Laboratory for personal use, not for redistribution. The definitive version was published in Biological Bulletin 209 (2005): 21-30.en
dc.description.abstractActivated Spisula oocytes proceed through meiotic stages rapidly and in near synchrony, providing an excellent system for analyzing polar body formation. Our previous studies suggested that cortical spreading of the metaphase peripheral aster determines spatial features of the cortical F-actin ring that is generated prior to extrusion of the polar body. We tested this hypothesis by experimentally altering the number and cortical contact patterns of peripheral asters. Such alteration was achieved by (a) lovastatin-induced arrest at metaphase I, with and without hexylene glycol modification, followed by washout; and (b) cytochalasin-D inhibition of extrusion of the first polar body, with washout before extrusion of the second polar body. Both methods induced simultaneous formation of two or more cortically spreading asters, correlated with subsequent formation of double, or even triple, overlapping F-actin rings during anaphase. Regardless of pattern, ring F-actin was deposited near regions of greatest astral microtubule density, indicating that microtubules provided a positive stimulus to which the cortex responded indiscriminately. These results strongly support the proposed causal relationship between peripheral aster spreading and biogenesis of the F-actin ring involved in polar body formation.en
dc.description.sponsorshipWe are indebted to the Howard Hughes Medical Institute Undergraduate Science Education Program in Biology (HHMI 5200267), the Hunter College Avon-Tukman Fund, NSF 9808368, and PSC-CUNY 65218, for support.en
dc.format.extent29239931 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen
dc.publisherMarine Biological Laboratoryen
dc.relation.urihttp://www.biolbull.org/content/209/1/21.abstract
dc.titlePolar body formation in Spisula oocytes : function of the peripheral asteren
dc.typeArticleen


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