Polar body formation in Spisula oocytes : function of the peripheral aster


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dc.contributor.author Pielak, Rafal M.
dc.contributor.author Hawkins, Christopher
dc.contributor.author Pyie, Aung
dc.contributor.author Bautista, Jennifer
dc.contributor.author Lee, Kyeng-Gea
dc.contributor.author Cohen, William D.
dc.date.accessioned 2006-10-04T20:21:45Z
dc.date.available 2006-10-04T20:21:45Z
dc.date.issued 2005-08
dc.identifier.citation Biological Bulletin 209 (2005): 21-30 en
dc.identifier.uri http://hdl.handle.net/1912/1260
dc.description Author Posting. © Marine Biological Laboratory, 2005. This article is posted here by permission of Marine Biological Laboratory for personal use, not for redistribution. The definitive version was published in Biological Bulletin 209 (2005): 21-30. en
dc.description.abstract Activated Spisula oocytes proceed through meiotic stages rapidly and in near synchrony, providing an excellent system for analyzing polar body formation. Our previous studies suggested that cortical spreading of the metaphase peripheral aster determines spatial features of the cortical F-actin ring that is generated prior to extrusion of the polar body. We tested this hypothesis by experimentally altering the number and cortical contact patterns of peripheral asters. Such alteration was achieved by (a) lovastatin-induced arrest at metaphase I, with and without hexylene glycol modification, followed by washout; and (b) cytochalasin-D inhibition of extrusion of the first polar body, with washout before extrusion of the second polar body. Both methods induced simultaneous formation of two or more cortically spreading asters, correlated with subsequent formation of double, or even triple, overlapping F-actin rings during anaphase. Regardless of pattern, ring F-actin was deposited near regions of greatest astral microtubule density, indicating that microtubules provided a positive stimulus to which the cortex responded indiscriminately. These results strongly support the proposed causal relationship between peripheral aster spreading and biogenesis of the F-actin ring involved in polar body formation. en
dc.description.sponsorship We are indebted to the Howard Hughes Medical Institute Undergraduate Science Education Program in Biology (HHMI 5200267), the Hunter College Avon-Tukman Fund, NSF 9808368, and PSC-CUNY 65218, for support. en
dc.format.extent 29239931 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US en
dc.publisher Marine Biological Laboratory en
dc.relation.uri http://www.biolbull.org/content/209/1/21.abstract
dc.title Polar body formation in Spisula oocytes : function of the peripheral aster en
dc.type Article en

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