Testosterone metabolism in Neomysis integer following exposure to benzo(a)pyrene

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Date
2006-03-31Author
Poelmans, S.
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Verslycke, Tim A.
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Monteyne, E.
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Noppe, H.
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Verheyden, K.
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Janssen, Colin R.
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De Brabander, H. F.
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https://hdl.handle.net/1912/1220As published
https://doi.org/10.1016/j.cbpb.2006.04.001Keyword
Benzo(a)pyrene; Biomarker; Cytochrome P450 modulator; Neomysis integer; Testosterone metabolismAbstract
Cytochromes P450 (CYPs) are important enzymes involved in the regulation of hormone
synthesis and in the detoxification and/or activation of xenobiotics. CYPs are found in
virtually all organisms, from archae, and eubacteria to eukaryota. A number of endocrine disruptors are suspected of exerting their effects through disruption of normal CYP function. Consequently, alterations in steroid hormone metabolism through changes in CYP could provide an important tool to evaluate potential effects of endocrine disruptors. The aim of this
study was to investigate the potential effects of the known CYP modulator, benzo(a)pyrene (B(a)P), on the testosterone metabolism in the invertebrate Neomysis integer (Crustacea; Mysidacea). N. integer were exposed for 96h to 0.43, 2.39, 28.83, 339.00 and 1682.86μg B(a)P L-1 and a solventcontrol, and subsequently their ability to metabolize testosterone was
assessed. Identification and quantification of the produced phase I and phase II testosterone metabolites was performed using liquid chromatography coupled with multiple mass spectrometry (LC-MS2). Significant changes were observed in the overall ability of N. integer to metabolize testosterone when exposed to 2.39, 28.83, 339.00 and 1682.86μg B(a)P L-1 as compared to the control animals.
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Author Posting. © Elsevier B.V., 2006. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 144 (2006): 405-412, doi:10.1016/j.cbpb.2006.04.001.
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Preprint: Poelmans, S., Verslycke, Tim A., Monteyne, E., Noppe, H., Verheyden, K., Janssen, Colin R., De Brabander, H. F., "Testosterone metabolism in Neomysis integer following exposure to benzo(a)pyrene", 2006-03-31, https://doi.org/10.1016/j.cbpb.2006.04.001, https://hdl.handle.net/1912/1220Related items
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