Now showing items 51-60 of 146
Reconstitution of dynamic microtubules with Drosophila XMAP215, EB1, and Sentin
(Rockefeller University Press, 2012-11-26)
Dynamic microtubules (MTs) are essential for various intracellular events, such as mitosis. In Drosophila melanogaster S2 cells, three MT tip-localizing proteins, Msps/XMAP215, EB1, and Sentin (an EB1 cargo protein), have ...
Next-generation transgenic mice for optogenetic analysis of neural circuits
(Frontiers Media, 2013-11-26)
Here we characterize several new lines of transgenic mice useful for optogenetic analysis of brain circuit function. These mice express optogenetic probes, such as enhanced halorhodopsin or several different versions of ...
Morphometric analysis of human embryonic stem cell-derived ventricular cardiomyocytes : determining the maturation state of a population by quantifying parameters in individual cells
(Hindawi Publishing Corp, 2015-07)
Quantitative methods were established to determine the level of maturation of human embryonic stem cell-derived ventricular cardiomyocytes (hESC-vCMs) that were treated with different metabolic stimulants (i.e., isoproterenol ...
Clustering of a kinesin-14 motor enables processive retrograde microtubule-based transport in plants
The molecular motors kinesin and dynein drive bidirectional motility along microtubules (MTs) in most eukaryotic cells1,2. Land plants, however, are a notable exception, since they contain a large number of kinesins but ...
The homeodomain transcription factor Phox2 in the stellate ganglion of the squid Loligo pealei
(The Company of Biologists, 2015-06-26)
Homeodomain transcription factors regulate development of embryos and cellular physiology in adult systems. Paired-type homeodomain genes constitute a subclass that has been particularly implicated in establishment of ...
Fluid interactions that enable stealth predation by the upstream-foraging hydromedusa Craspedacusta sowerbyi
(Marine Biological Laboratory, 2013-09-01)
Unlike most medusae that forage with tentacles trailing behind their bells, several species forage upstream of their bells using aborally located tentacles. It has been hypothesized that these medusae forage as stealth ...
Superfast vocal muscles control song production in songbirds
(Public Library of Science, 2008-07-09)
Birdsong is a widely used model for vocal learning and human speech, which exhibits high temporal and acoustic diversity. Rapid acoustic modulations are thought to arise from the vocal organ, the syrinx, by passive ...
Bilaterian phylogenetic trees
Influence of acidic pH on hydrogen and acetate production by an electrosynthetic microbiome
(Public Library of Science, 2014-10-15)
Production of hydrogen and organic compounds by an electrosynthetic microbiome using electrodes and carbon dioxide as sole electron donor and carbon source, respectively, was examined after exposure to acidic pH (~5). ...
Myosin5a tail associates directly with Rab3A-containing compartments in neurons
Myosin-Va (Myo5a) is a motor protein associated with synaptic vesicles (SVs) but the mechanism by which it interacts has not yet been identified. A potential class of binding partners are Rab GTPases and Rab3A is ...