• Login
    About WHOAS
    View Item 
    •   WHOAS Home
    • Woods Hole Oceanographic Institution
    • Biology
    • View Item
    •   WHOAS Home
    • Woods Hole Oceanographic Institution
    • Biology
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of WHOASCommunities & CollectionsBy Issue DateAuthorsTitlesKeywordsThis CollectionBy Issue DateAuthorsTitlesKeywords

    My Account

    LoginRegister

    Statistics

    View Usage Statistics

    Isolation and phylogeny of novel cytochrome P450 genes from tunicates (Ciona spp.) : a CYP3 line in early deuterostomes?

    Thumbnail
    View/Open
    Author's final draft (84.92Kb)
    Figure legends (11.75Kb)
    Figure 1 (14.86Kb)
    Figure 2 (19.56Kb)
    Figure 3 (14.15Kb)
    Figure 4 (16.64Kb)
    Table 1 (14.13Kb)
    Table 2 (79.40Kb)
    Table 3 (19.54Kb)
    Supplementary material: Figure 1 (15.36Kb)
    Supplementary material: Table 1 (30.93Kb)
    Supplementary material: Table 2 (12.86Kb)
    Supplementary material: Table 3 (21.74Kb)
    Date
    2006-04-04
    Author
    Verslycke, Tim A.  Concept link
    Goldstone, Jared V.  Concept link
    Stegeman, John J.  Concept link
    Metadata
    Show full item record
    Citable URI
    https://hdl.handle.net/1912/1133
    As published
    https://doi.org/10.1016/j.ympev.2006.04.017
    Keyword
     Ciona intestinalis; Ciona savignyi; Cytochrome P450; CYP3; Phylogeny; Nomenclature 
    Abstract
    Cytochromes P450 (CYPs) form a gene superfamily involved in the biotransformation of numerous endogenous and exogenous natural and synthetic compounds. In humans, CYP3A4 is regarded as one of the most important CYPs due to its abundance in liver and its capacity to metabolize more than 50% of all clinically used drugs. It has been suggested that all CYP3s arose from a common ancestral gene lineage that diverged between 800 and 1100 million years ago, before the deuterostome-protostome split. While CYP3s are well known in mammals and have been described in lower vertebrates, they have not been reported in non-vertebrate deuterostomes. Members of the genus Ciona belong to the tunicates, whose lineage is thought to be the most basal among the chordates, and from which the vertebrate line diverged. Here we describe the cloning, exon-intron structure, phylogeny, and estimated expression of four novel genes from Ciona intestinalis. We also describe the gene structure and phylogeny of homologous genes in Ciona savignyi. Comparing these genes with other members of the CYP clan 3, show that the Ciona sequences bear remarkable similarity to vertebrate CYP3A genes, and may be an early deuterostome CYP3 line.
    Description
    Author Posting. © Elsevier B.V., 2006. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Molecular Phylogenetics and Evolution 40 (2006): 760-771, doi:10.1016/j.ympev.2006.04.017.
    Collections
    • Biology
    Suggested Citation
    Preprint: Verslycke, Tim A., Goldstone, Jared V., Stegeman, John J., "Isolation and phylogeny of novel cytochrome P450 genes from tunicates (Ciona spp.) : a CYP3 line in early deuterostomes?", 2006-04-04, https://doi.org/10.1016/j.ympev.2006.04.017, https://hdl.handle.net/1912/1133
     

    Related items

    Showing items related by title, author, creator and subject.

    • Thumbnail

      Involvement of Cytochrome P450 1A in the toxicity of aryl hydrocarbon receptor agonists : alteration arachidonic acid metabolism and production of reactive oxygen species 

      Schlezinger, Jennifer J. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 1998-08)
      Two cytochrome P4501A-dependent mechanisms of aryl hydrocarbon receptor (AhR) agonist toxicity were examined in the marine teleost scup (Stenotomus chrysops), alteration of arachidonic acid (AA) metabolism and production ...
    • Thumbnail

      Diversity and characterization of novel Cytochrome P450 2 genes in the marine teleost Fundulus Heteroclitus 

      Oleksiak, Marjorie F. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 1997-09)
      The multiplicity and roles of cytochrome P450 CYP genes in the 2 family are not well known in fish. Characterizations of CYP2 genes were done in the killifish, Fundulus heteroclitus. Multiple cDNAs from three CYP2 ...
    • Thumbnail

      Regeneration of oral siphon pigment organs in the ascidian Ciona intestinalis 

      Auger, Helene; Sasakura, Yasunori; Joly, Jean-Stephane; Jeffery, William R. (2009-12-06)
      Ascidians have powerful capacities for regeneration but the underlying mechanisms are poorly understood. Here we examine oral siphon regeneration in the solitary ascidian Ciona intestinalis. Following amputation, the ...
    All Items in WHOAS are protected by original copyright, with all rights reserved, unless otherwise indicated. WHOAS also supports the use of the Creative Commons licenses for original content.
    A service of the MBLWHOI Library | About WHOAS
    Contact Us | Send Feedback | Privacy Policy
    Core Trust Logo