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dc.contributor.authorAllard, Corey A. H.  Concept link
dc.contributor.authorDecker, Franziska  Concept link
dc.contributor.authorWeiner, Orion  Concept link
dc.contributor.authorToettcher, Jared E.  Concept link
dc.contributor.authorGraziano, Brian R.  Concept link
dc.date.accessioned2019-01-22T21:13:52Z
dc.date.available2019-01-22T21:13:52Z
dc.date.issued2018-12-21
dc.identifier.citationAllard CAH, Decker F, Weiner OD, Toettcher JE, Graziano BR (2018) A size-invariant bud-duration timer enables robustness in yeast cell size control. PLoS ONE 13(12): e0209301en_US
dc.identifier.urihttps://hdl.handle.net/1912/10833
dc.description© The Author(s), 2018. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLoS One 13(12), (2018): e0209301, doi:0.1371/journal.pone.0140578.en_US
dc.description.abstractCell populations across nearly all forms of life generally maintain a characteristic cell type-dependent size, but how size control is achieved has been a long-standing question. The G1/S boundary of the cell cycle serves as a major point of size control, and mechanisms operating here restrict passage of cells to Start if they are too small. In contrast, it is less clear how size is regulated post-Start, during S/G2/M. To gain further insight into post-Start size control, we prepared budding yeast that can be reversibly blocked from bud initiation. While blocked, cells continue to grow isotropically, increasing their volume by more than an order of magnitude over unperturbed cells. Upon release from their block, giant mothers reenter the cell cycle and their progeny rapidly return to the original unperturbed size. We found this behavior to be consistent with a size-invariant ‘timer’ specifying the duration of S/G2/M. These results indicate that yeast use at least two distinct mechanisms at different cell cycle phases to ensure size homeostasis.en_US
dc.description.sponsorshipSupport for this work was provided by a Post-Physiology Course Award from the Marine Biological Laboratory to C.A.H.A., the Thomas B. Grave and Elizabeth F. Grave Scholarship to F.D., and the National Institutes of Health [GM118167 to O.D.W., DP2EB024247 to J.E.T., and T32HL773125 to B.R.G.]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. There was no additional external funding received for this study.en_US
dc.relation.urihttps://doi.org/10.1371/journal.pone.0209301
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleA size-invariant bud-duration timer enables robustness in yeast cell size controlen_US
dc.typeArticleen_US
dc.identifier.doi10.1371/journal.pone.0209301


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International