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    Transcriptomic and proteomic insight into the mechanism of cyclooctasulfur‐ versus thiosulfate‐oxidation by the chemolithoautotroph Sulfurimonas denitrificans

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    Date
    2018-10
    Author
    Götz, Florian  Concept link
    Pjevac, Petra  Concept link
    Markert, Stephanie  Concept link
    McNichol, Jesse C.  Concept link
    Becher, Dorte  Concept link
    Schweder, Thomas  Concept link
    Mussmann, Marc  Concept link
    Sievert, Stefan M.  Concept link
    Metadata
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    Citable URI
    https://hdl.handle.net/1912/10803
    As published
    https://doi.org/10.1111/1462-2920.14452
    Abstract
    Chemoautotrophic bacteria belonging to the genus Sulfurimonas (class Campylobacteria) were previously identified as key players in the turnover of zero‐valence sulfur, a central intermediate in the marine sulfur cycle. S. denitrificans was further shown to be able to oxidize cyclooctasulfur (S8). However, at present the mechanism of activation and metabolism of cyclooctasulfur is not known. Here, we assessed the transcriptome and proteome of S. denitrificans grown with either thiosulfate or S8 as the electron donor. While the overall expression profiles under the two growth conditions were rather similar, distinct differences were observed that could be attributed to the utilization of S8. This included a higher abundance of expressed genes related to surface attachment in the presence of S8, and the differential regulation of the sulfur‐oxidation multienzyme complex (SOX), which in S. denitrificans is encoded in two gene clusters: soxABXY 1Z 1 and soxCDY 2Z 2. While the proteins of both clusters were present with thiosulfate, only proteins of the soxCDY 2Z 2 were detected at significant levels with S8. Based on these findings a model for the oxidation of S8 is proposed. Our results have implications for interpreting metatranscriptomic and ‐proteomic data and for the observed high level of diversification of soxY 2Z 2 among sulfur‐oxidizing Campylobacteria.
    Description
    Author Posting. © The Author(s), 2018. This is the author's version of the work. It is posted here under a nonexclusive, irrevocable, paid-up, worldwide license granted to WHOI. It is made available for personal use, not for redistribution. The definitive version was published in Environmental Microbology, (2018),doi:.10.1111/1462-2920.14452
    Collections
    • Biology
    Suggested Citation
    Preprint: Götz, Florian, Pjevac, Petra, Markert, Stephanie, McNichol, Jesse C., Becher, Dorte, Schweder, Thomas, Mussmann, Marc, Sievert, Stefan M., "Transcriptomic and proteomic insight into the mechanism of cyclooctasulfur‐ versus thiosulfate‐oxidation by the chemolithoautotroph Sulfurimonas denitrificans", 2018-10, https://doi.org/10.1111/1462-2920.14452, https://hdl.handle.net/1912/10803
     
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