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dc.contributor.authorSerres, Margrethe H.  Concept link
dc.contributor.authorGopal, Shuba  Concept link
dc.contributor.authorNahum, Laila A.  Concept link
dc.contributor.authorLiang, Ping  Concept link
dc.contributor.authorGaasterland, Terry  Concept link
dc.contributor.authorRiley, Monica  Concept link
dc.date.accessioned2005-11-14T20:51:47Z
dc.date.available2005-11-14T20:51:47Z
dc.date.issued2001-08-20
dc.identifier.citationGenome Biology 2 (2001): research0035.1–0035.7en
dc.identifier.urihttps://hdl.handle.net/1912/106
dc.descriptionAuthor Posting. © 2001 Serres et al. The definitive version was published in Genome Biology 2 (2001): research0035.1–0035.7, doi:10.1186/gb-2001-2-9-research0035.
dc.description.abstractBackground: Since the genome of Escherichia coli K-12 was initially annotated in 1997, additional functional information based on biological characterization and functions of sequence-similar proteins has become available. On the basis of this new information, an updated version of the annotated chromosome has been generated. Results: The E. coli K-12 chromosome is currently represented by 4,401 genes encoding 116 RNAs and 4,285 proteins. The boundaries of the genes identified in the GenBank Accession U00096 were used. Some protein-coding sequences are compound and encode multimodular proteins. The coding sequences (CDSs) are represented by modules (protein elements of at least 100 amino acids with biological activity and independent evolutionary history). There are 4,616 identified modules in the 4,285 proteins. Of these, 48.9% have been characterized, 29.5% have an imputed function, 2.1% have a phenotype and 19.5% have no function assignment. Only 7% of the modules appear unique to E. coli, and this number is expected to be reduced as more genome data becomes available. The imputed functions were assigned on the basis of manual evaluation of functions predicted by BLAST and DARWIN analyses and by the MAGPIE genome annotation system. Conclusions: Much knowledge has been gained about functions encoded by the E. coli K-12 genome since the 1997 annotation was published. The data presented here should be useful for analysis of E. coli gene products as well as gene products encoded by other genomes.en
dc.description.sponsorshipThis work was supported by NIH grant RO1 RR07861, the NASA Astrobiology Institute grant NCC2-1054, grants from the Edward Mallinckrodt, Jr Foundation and the Sinsheimer Foundation, and NSF grants NSF DBI - 9984882 and NSF IIS - 9996304.en
dc.format.extent89280 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen
dc.publisherBioMed Centralen
dc.relation.urihttps://doi.org/10.1186/gb-2001-2-9-research0035
dc.subjectEscherichia coli K-12en
dc.titleA functional update of the Escherichia coli K-12 genomeen
dc.typeArticleen
dc.identifier.doi10.1186/gb-2001-2-9-research0035


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