The pleiotropic effects of the glutamate dehydrogenase (GDH) pathway in Saccharomyces cerevisiae
Fragiadakis, G. S.
MetadataShow full item record
KeywordGlutamate dehydrogenase; GDH1; GDH2; GDH3; Ammonium assimilation; GABA shunt; ROS-mediated apoptosis; Chromatin regulation; Nitrogen catabolite repression; S. cerevisiae
Ammonium assimilation is linked to fundamental cellular processes that include the synthesis of non-essential amino acids like glutamate and glutamine. In Saccharomyces cerevisiae glutamate can be synthesized from α-ketoglutarate and ammonium through the action of NADP-dependent glutamate dehydrogenases Gdh1 and Gdh3. Gdh1 and Gdh3 are evolutionarily adapted isoforms and cover the anabolic role of the GDH-pathway. Here, we review the role and function of the GDH pathway in glutamate metabolism and we discuss the additional contributions of the pathway in chromatin regulation, nitrogen catabolite repression, ROS-mediated apoptosis, iron deficiency and sphingolipid-dependent actin cytoskeleton modulation in S.cerevisiae. The pleiotropic effects of GDH pathway in yeast biology highlight the importance of glutamate homeostasis in vital cellular processes and reveal new features for conserved enzymes that were primarily characterized for their metabolic capacity. These newly described features constitute insights that can be utilized for challenges regarding genetic engineering of glutamate homeostasis and maintenance of redox balances, biosynthesis of important metabolites and production of organic substrates. We also conclude that the discussed pleiotropic features intersect with basic metabolism and set a new background for further glutamate-dependent applied research of biotechnological interest.
© The Author(s), 2018. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Microbial Cell Factories 17 (2018): 170, doi:10.1186/s12934-018-1018-4.
Suggested CitationMicrobial Cell Factories 17 (2018): 170
The following license files are associated with this item:
Showing items related by title, author, creator and subject.
Modulation of extracellular proton fluxes from retinal horizontal cells of the catfish by depolarization and glutamate Kreitzer, Matthew A.; Collis, Leon P.; Molina, Anthony J. A.; Smith, Peter J. S.; Malchow, Robert Paul (Rockefeller University Press, 2007-07-30)Self-referencing H+-selective microelectrodes were used to measure extracellular proton fluxes from cone-driven horizontal cells isolated from the retina of the catfish (Ictalurus punctatus). The neurotransmitter glutamate ...
Palmitoylation regulates glutamate receptor distributions in postsynaptic densities through control of PSD95 conformation and orientation Jeyifous, Okunola; Lin, Eric I.; Chen, Xiaobing; Antinone, Sarah E.; Mastro, Ryan; Drisdel, Renaldo; Reese, Thomas S.; Green, William N. (2016-08)PSD95 and SAP97 are homologous scaffold proteins with different N-terminal domains, possessing either a palmitoylation site (PSD95) or an L27 domain (SAP97). Here, we measured PSD95 and SAP97 conformation in vitro and ...
N isotopic composition of Phenylalanine and Glutamic Acid from a number of organisms, demonstrating new HPLC protocol for precise isotopic measurements McCarthy, Matthew D. (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: firstname.lastname@example.org, 2019-08-02)N isotopic composition of Phenylalanine and Glutamic Acid from a number of organisms, demonstrating new HPLC protocol for precise isotopic measurements. For a complete list of measurements, refer to the full dataset ...