Microbial biogeography along an estuarine salinity gradient : combined influences of bacterial growth and residence time


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dc.contributor.author Crump, Byron C.
dc.contributor.author Hopkinson, Charles S.
dc.contributor.author Sogin, Mitchell L.
dc.contributor.author Hobbie, John E.
dc.date.accessioned 2005-11-14T20:21:14Z
dc.date.available 2005-11-14T20:21:14Z
dc.date.issued 2004-03
dc.identifier.citation Applied and Environmental Microbiology 70 (2004): 1494-1505 en
dc.identifier.uri http://hdl.handle.net/1912/105
dc.description Author Posting. © American Society for Microbiology, 2004. This article is posted here by permission of American Society for Microbiology for personal use, not for redistribution. The definitive version was published in Applied and Environmental Microbiology 70 (2004): 1494-1505, doi:10.1128/AEM.70.3.1494-1505.2004.
dc.description.abstract Shifts in bacterioplankton community composition along the salinity gradient of the Parker River estuary and Plum Island Sound, in northeastern Massachusetts, were related to residence time and bacterial community doubling time in spring, summer, and fall seasons. Bacterial community composition was characterized with denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S ribosomal DNA. Average community doubling time was calculated from bacterial production ([14C]leucine incorporation) and bacterial abundance (direct counts). Freshwater and marine populations advected into the estuary represented a large fraction of the bacterioplankton community in all seasons. However, a unique estuarine community formed at intermediate salinities in summer and fall, when average doubling time was much shorter than water residence time, but not in spring, when doubling time was similar to residence time. Sequencing of DNA in DGGE bands demonstrated that most bands represented single phylotypes and that matching bands from different samples represented identical phylotypes. Most river and coastal ocean bacterioplankton were members of common freshwater and marine phylogenetic clusters within the phyla Proteobacteria, Bacteroidetes, and Actinobacteria. Estuarine bacterioplankton also belonged to these phyla but were related to clones and isolates from several different environments, including marine water columns, freshwater sediments, and soil. en
dc.description.sponsorship This work was supported by two grants from the National Science Foundation (LTER grant OCE-9726921 and Microbial Observatory grant MCB-9977897) and the NASA Astrobiology Institute (cooperative agreement NCC2-1054 to M.L.S.). en
dc.format.extent 3341462 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US en
dc.publisher American Society for Microbiology en
dc.relation.uri http://dx.doi.org/10.1128/AEM.70.3.1494-1505.2004
dc.subject Bacterioplankton community composition en
dc.subject Parker River estuary en
dc.subject Plum Island Sound en
dc.subject Proteobacteria en
dc.subject Bacteroidetes en
dc.subject Actinobacteria en
dc.title Microbial biogeography along an estuarine salinity gradient : combined influences of bacterial growth and residence time en
dc.type Article en
dc.identifier.doi 10.1128/AEM.70.3.1494-1505.2004

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