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dc.contributor.authorOh, Dae-Ju  Concept link
dc.contributor.authorHur, Sung-Pyo  Concept link
dc.contributor.authorBouchekioua, Selma  Concept link
dc.contributor.authorTakeuchi, Yuki  Concept link
dc.contributor.authorUdagawa, Shingo  Concept link
dc.contributor.authorAluru, Neelakanteswar  Concept link
dc.contributor.authorPark, Yong-Ju  Concept link
dc.contributor.authorPark, Ji-Gweon  Concept link
dc.contributor.authorKim, Se-Jae  Concept link
dc.contributor.authorMoon, Thomas W.  Concept link
dc.contributor.authorVijayan, Mathilakath M.  Concept link
dc.contributor.authorTakemura, Akihiro  Concept link
dc.date.accessioned2018-07-31T16:50:14Z
dc.date.available2018-07-31T16:50:14Z
dc.date.issued2018-05
dc.identifier.urihttps://hdl.handle.net/1912/10504
dc.descriptionAuthor Posting. © The Author(s), 2018. This is the author's version of the work. It is posted here under a nonexclusive, irrevocable, paid-up, worldwide license granted to WHOI. It is made available for personal use, not for redistribution. The definitive version was published in Ocean Science Journal 53 (2018): 239-249, doi:10.1007/s12601-018-0016-0.en_US
dc.description.abstractThe threespot wrasse (Halichoeres trimaculatus; Family Labridae) is a common coral reef species of the Indo-Pacific Ocean. Given that this species spawns daily at high tide (HT), we hypothesized that endocrine changes in relation to gonadal development are synchronized with the tidal cycle. To test this, we examined the transcript abundance of two cytochrome P450 aromatases (cyp19a and cyp19b) and two estrogen receptors (er and er) in the ovary and brain of this species in response to tidal change. When fish were collected around four tidal points [low tide (LT), flood tide (FT), high tide (HT), and ebb tide (ET)], gonadosomatic index and oocyte diameter increased around HT and FT, respectively. Ovulatory follicles were observed in ovaries around HT. Real-time quantitative polymerase-chain reaction revealed that mRNA abundance of cyp19a and er, but not er, in the ovary increased around ET and HT, respectively. On the other hand, mRNA levels of cyp19b in the forebrain were significantly higher around FT. Increases of er and er mRNA abundance around FT were observed in all areas of the brain and the midbrain, respectively. The changes in mRNA abundance of key genes involved in reproduction at specific tidal cycles, along with the development of the vitellogenic oocytes in the ovary, support our hypothesis that synchronization of endocrine changes to the tidal periodicity plays a role in the gonadal development of this species. We hypothesize that conversion of testosterone to E2 in the brain may be associated with the spawning behavior given that the wrasse exhibits group spawning with a territory-holding male around HT.en_US
dc.description.sponsorshipThis study was supported partially by the 21st Century COE program “The Comprehensive Analyses on Biodiversity in Coral Reef and Island Ecosystems in Asian and Pacific Regions” from the Ministry of Education, Culture, Sports, Science and Technology, Japan, JSPS KAKENHI Grant number 16H05796, and the Natural Sciences and Engineering Research Council (NSERC, Canada) Discovery Grant program.en_US
dc.language.isoen_USen_US
dc.relation.urihttps://doi.org/10.1007/s12601-018-0016-0
dc.subjectAromataseen_US
dc.subjectBrainen_US
dc.subjectEstrogen receptoren_US
dc.subjectOvaryen_US
dc.subjectTideen_US
dc.subjectWrasseen_US
dc.titleTide-related changes in mRNA abundance of aromatases and estrogen receptors in the ovary and brain of the threespot wrasse Halichoeres trimaculatusen_US
dc.typePreprinten_US


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