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    Microbial biogeography along an estuarine salinity gradient : combined influences of bacterial growth and residence time

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    Crump et al.pdf (3.186Mb)
    Date
    2004-03
    Author
    Crump, Byron C.  Concept link
    Hopkinson, Charles S.  Concept link
    Sogin, Mitchell L.  Concept link
    Hobbie, John E.  Concept link
    Metadata
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    Citable URI
    https://hdl.handle.net/1912/105
    As published
    https://doi.org/10.1128/AEM.70.3.1494-1505.2004
    DOI
    10.1128/AEM.70.3.1494-1505.2004
    Keyword
     Bacterioplankton community composition; Parker River estuary; Plum Island Sound; Proteobacteria; Bacteroidetes; Actinobacteria 
    Abstract
    Shifts in bacterioplankton community composition along the salinity gradient of the Parker River estuary and Plum Island Sound, in northeastern Massachusetts, were related to residence time and bacterial community doubling time in spring, summer, and fall seasons. Bacterial community composition was characterized with denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S ribosomal DNA. Average community doubling time was calculated from bacterial production ([14C]leucine incorporation) and bacterial abundance (direct counts). Freshwater and marine populations advected into the estuary represented a large fraction of the bacterioplankton community in all seasons. However, a unique estuarine community formed at intermediate salinities in summer and fall, when average doubling time was much shorter than water residence time, but not in spring, when doubling time was similar to residence time. Sequencing of DNA in DGGE bands demonstrated that most bands represented single phylotypes and that matching bands from different samples represented identical phylotypes. Most river and coastal ocean bacterioplankton were members of common freshwater and marine phylogenetic clusters within the phyla Proteobacteria, Bacteroidetes, and Actinobacteria. Estuarine bacterioplankton also belonged to these phyla but were related to clones and isolates from several different environments, including marine water columns, freshwater sediments, and soil.
    Description
    Author Posting. © American Society for Microbiology, 2004. This article is posted here by permission of American Society for Microbiology for personal use, not for redistribution. The definitive version was published in Applied and Environmental Microbiology 70 (2004): 1494-1505, doi:10.1128/AEM.70.3.1494-1505.2004.
    Collections
    • Ecosystems Center
    • Josephine Bay Paul Center in Comparative Molecular Biology and Evolution
    Suggested Citation
    Applied and Environmental Microbiology 70 (2004): 1494-1505
     
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