The effect of sample drying temperature on marine particulate organic carbon composition
Rosengard, Sarah Z.
Lam, Phoebe J.
McNichol, Ann P.
Johnson, Carl G.
MetadataShow full item record
Compositional changes in marine particulate organic carbon (POC) throughout the water column trace important processes that underlie the biological pump’s efficiency. While labor-intensive, particle sampling efforts offer potential to expand the empirical POC archive at different stages in the water column, provided that organic composition is sufficiently preserved between sampling and analysis. The standard procedure for preserving organic matter composition in marine samples is to immediately store particles at -80°C to -20°C until they can be freeze-dried for analysis. This report investigates the effect of warmer drying and storage temperatures on POC composition, which applies to the majority of POC samples collected in the field without intention for organic analysis. Particle samples collected off Woods Hole, MA were immediately dried at 56°C, at room temperature, or stored at -80°C until being freeze-dried. Results show that oven- and air-drying did not shift the bulk composition (i.e., carbon and nitrogen content and stable isotope composition) of POC in the samples relative to freeze-drying. Similarly, warmer drying temperatures did not affect POC thermal stability, as inferred by ramped pyrolysis/oxidation (RPO), a growing technique that uses a continuous temperature ramp to differentiate components of organic carbon by their decomposition temperature. Oven- and air-drying did depress lipid abundances relative to freeze-drying, the extent of which depended on compound size and structure. The data suggest that field samples dried at room temperatures and 56°C are appropriate for assessing bulk POC composition and thermal stability, but physical mechanisms such as molecular volatilization bias their lipid composition.
Author Posting. © The Author(s), 2018. This is the author's version of the work. It is posted here under a nonexclusive, irrevocable, paid-up, worldwide license granted to WHOI. It is made available for personal use, not for redistribution. The definitive version was published in Limnology and Oceanography Methods 16 (2018): 286-298, doi:10.1002/lom3.10245.
Suggested CitationPreprint: Rosengard, Sarah Z., Lam, Phoebe J., McNichol, Ann P., Johnson, Carl G., Galy, Valier, "The effect of sample drying temperature on marine particulate organic carbon composition", 2018-02, https://doi.org/10.1002/lom3.10245, https://hdl.handle.net/1912/10459
Showing items related by title, author, creator and subject.
Seasonal hydrology drives rapid shifts in the flux and composition of dissolved and particulate organic carbon and major and trace ions in the Fraser River, Canada Voss, Britta M.; Peucker-Ehrenbrink, Bernhard; Eglinton, Timothy I.; Spencer, Robert G. M.; Bulygina, Ekaterina; Galy, Valier; Lamborg, Carl H.; Ganguli, Priya M.; Montlucon, Daniel B.; Marsh, Steve; Gillies, Sharon L.; Fanslau, Jenna; Epp, A.; Luymes, Rosalie (Copernicus Publications on behalf of the European Geosciences Union, 2015-10-01)Rapid changes in the volume and sources of discharge during the spring freshet lead to pronounced variations in biogeochemical properties in snowmelt-dominated river basins. We used daily sampling during the onset of the ...
Primary production of particulate protein amino acids : algal protein metabolism and its relationship to the composition of particulate organic matter Lohrenz, Steven E. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 1985-04)The biochemical and physiological bases underlying primary production of particulate protein amino acids were investigated in an effort to understand the relationship between algal protein metabolism and particulate organic ...
An assessment of particulate organic carbon to thorium-234 ratios in the ocean and their impact on the application of 234Th as a POC flux proxy Buesseler, Ken O.; Benitez-Nelson, Claudia R.; Burd, Adrian B.; Charette, Matthew A.; Cochran, J. Kirk; Coppola, L.; Fisher, Nicholas S.; Fowler, Scott W.; Gardner, Wilford D.; Guo, L. D.; Gustafsson, Orjan; Lamborg, Carl H.; Masqué, Pere; Miquel, Juan Carlos; Passow, Uta; Santschi, Peter H.; Savoye, Nicolas; Stewart, G.; Trull, Thomas W. (2005-06-18)Thorium-234 is increasingly used as a tracer of ocean particle flux, primarily as a means to estimate particulate organic carbon export from the surface ocean. This requires determination of both the 234Th activity ...