Oxidative formation and removal of complexed Mn(III) by Pseudomonas species
Date
2018-04-12Author
Wright, Mitchell H.
Concept link
Geszvain, Kati
Concept link
Oldham, Véronique E.
Concept link
Luther, George W.
Concept link
Tebo, Bradley M.
Concept link
Metadata
Show full item recordCitable URI
https://hdl.handle.net/1912/10306As published
https://doi.org/10.3389/fmicb.2018.00560DOI
10.3389/fmicb.2018.00560Keyword
Manganese(III); Mn(III)-DFOB; Mn(III)-citrate; Mn(III)-L; Pseudomonas; Bacterial manganese oxidationAbstract
The observation of significant concentrations of soluble Mn(III) complexes in oxic, suboxic, and some anoxic waters has triggered a re-evaluation of the previous Mn paradigm which focused on the cycling between soluble Mn(II) and insoluble Mn(III,IV) species as operationally defined by filtration. Though Mn(II) oxidation in aquatic environments is primarily bacterially-mediated, little is known about the effect of Mn(III)-binding ligands on Mn(II) oxidation nor on the formation and removal of Mn(III). Pseudomonas putida GB-1 is one of the most extensively investigated of all Mn(II) oxidizing bacteria, encoding genes for three Mn oxidases (McoA, MnxG, and MopA). P. putida GB-1 and associated Mn oxidase mutants were tested alongside environmental isolates Pseudomonas hunanensis GSL-007 and Pseudomonas sp. GSL-010 for their ability to both directly oxidize weakly and strongly bound Mn(III), and to form these complexes through the oxidation of Mn(II). Using Mn(III)-citrate (weak complex) and Mn(III)-DFOB (strong complex), it was observed that P. putida GB-1, P. hunanensis GSL-007 and Pseudomonas sp. GSL-010 and mutants expressing only MnxG and McoA were able to directly oxidize both species at varying levels; however, no oxidation was detected in cultures of a P. putida mutant expressing only MopA. During cultivation in the presence of Mn(II) and citrate or DFOB, P. putida GB-1, P. hunanensis GSL-007 and Pseudomonas sp. GSL-010 formed Mn(III) complexes transiently as an intermediate before forming Mn(III/IV) oxides with the overall rates and extents of Mn(III,IV) oxide formation being greater for Mn(III)-citrate than for Mn(III)-DFOB. These data highlight the role of bacteria in the oxidative portion of the Mn cycle and suggest that the oxidation of strong Mn(III) complexes can occur through enzymatic mechanisms involving multicopper oxidases. The results support the observations from field studies and further emphasize the complexity of the geochemical cycling of manganese.
Description
© The Author(s), 2018. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Frontiers in Microbiology 9 (2018): 560, doi:10.3389/fmicb.2018.00560.
Collections
Suggested Citation
Frontiers in Microbiology 9 (2018): 560The following license files are associated with this item:
Related items
Showing items related by title, author, creator and subject.
-
A few Pseudomonas oligotypes dominate in the meat and dairy processing environment
Stellato, Giuseppina; Utter, Daniel R.; Voorhis, Andy; De Angelis, Maria; Eren, A. Murat; Ercolini, Danilo (Frontiers Media, 2017-03-02)The occurrence of bacteria in the food processing environments plays a key role in food contamination and development of spoilage. Species of the genus Pseudomonas are recognized as major food spoilers and the capability ... -
Better to light a candle than curse the darkness : illuminating spatial localization and temporal dynamics of rapid microbial growth in the rhizosphere
Herron, Patrick M.; Gage, Daniel J.; Pinedo, Catalina Arango; Haider, Zane K.; Cardon, Zoe G. (Frontiers Media, 2013-09-02)The rhizosphere is a hotbed of microbial activity in ecosystems, fueled by carbon compounds from plant roots. Basic questions about the location and dynamics of plant-spurred microbial growth in the rhizosphere are difficult ... -
The microbiome in pediatric cystic fibrosis patients : the role of shared environment suggests a window of intervention
Hampton, Thomas H.; Green, Deanna M.; Cutting, Garry R.; Morrison, Hilary G.; Sogin, Mitchell L.; Gifford, Alex H.; Stanton, Bruce A.; O’Toole, George A. (BioMed Central, 2014-04-28)Cystic fibrosis (CF) is caused by mutations in the CFTR gene that predispose the airway to infection. Chronic infection by pathogens such as Pseudomonas aeruginosa leads to inflammation that gradually degrades lung function, ...