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dc.contributor.authorCardon, Zoe G.  Concept link
dc.contributor.authorPeredo, Elena L.  Concept link
dc.contributor.authorDohnalkova, Alice C.  Concept link
dc.contributor.authorGershone, Hannah L.  Concept link
dc.contributor.authorBezanilla, Magdalena  Concept link
dc.date.accessioned2018-04-11T15:50:10Z
dc.date.issued2018-04-10
dc.identifier.citationJournal of Cell Science 131 (2018): jcs212233en_US
dc.identifier.urihttps://hdl.handle.net/1912/10223
dc.descriptionAuthor Posting. © The Company of Biologists, 2018. This article is posted here by permission of The Company of Biologists for personal use, not for redistribution. The definitive version was published in Journal of Cell Science 131 (2018): jcs212233, doi:10.1242/jcs.212233.en_US
dc.description.abstractMicroscopic green algae inhabiting desert microbiotic crusts are remarkably diverse phylogenetically, and many desert lineages have independently evolved from aquatic ancestors. Here we worked with five desert and aquatic species within the family Scenedesmaceae to examine mechanisms that underlie desiccation tolerance and release of unicellular versus multicellular progeny. Live cell staining and time-lapse confocal imaging coupled with transmission electron microscopy established that the desert and aquatic species all divide by multiple (rather than binary) fission, although progeny were unicellular in three species and multicellular (joined in a sheet-like coenobium) in two. During division, Golgi complexes were localized near nuclei, and all species exhibited dynamic rotation of the daughter cell mass within the mother cell wall at cytokinesis. Differential desiccation tolerance across the five species, assessed from photosynthetic efficiency during desiccation/rehydration cycles, was accompanied by differential accumulation of intracellular reactive oxygen species (ROS) detected using a dye sensitive to intracellular ROS. Further comparative investigation will aim to understand the genetic, ultrastructural and physiological characteristics supporting unicellular versus multicellular coenobial morphology, and the ability of representatives in the Scenedesmaceae to colonize ecologically diverse, even extreme, habitats.en_US
dc.description.sponsorshipThis work was supported by the National Science Foundation, Division of Integrative Organismal Systems [1355085 to Z.G.C.], an anonymous donor [to Z.G.C.], the Marine Biological Laboratory [to M.B.] and the Environmental and Molecular Sciences Laboratory (EMSL) [48938 to Z.G.C.], a Department of Energy, Office of Science User Facility sponsored by the Office of Biological and Environmental Research, located at Pacific Northwest National Laboratory.en_US
dc.language.isoen_USen_US
dc.publisherThe Company of Biologistsen_US
dc.relation.urihttps://doi.org/10.1242/jcs.212233
dc.subjectROSen_US
dc.subjectPhotosynthesisen_US
dc.subjectMultiple fissionen_US
dc.subjectScenedesmusen_US
dc.subjectEnallaxen_US
dc.subjectTetradesmusen_US
dc.titleA model suite of green algae within the Scenedesmaceae for investigating contrasting desiccation tolerance and morphologyen_US
dc.typeArticleen_US
dc.description.embargo2019-04-10en_US
dc.identifier.doi10.1242/jcs.212233
dc.embargo.liftdate2019-04-10


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