Inter-­comparison of the potentially active prokaryotic communities in the halocline sediments of Mediterranean deep-sea hypersaline basins

dc.contributor.author Kormas, Konstantinos Ar.
dc.contributor.author Pachiadaki, Maria G.
dc.contributor.author Karayanni, Hera
dc.contributor.author Leadbetter, Edward R.
dc.contributor.author Bernhard, Joan M.
dc.contributor.author Edgcomb, Virginia P.
dc.date.accessioned 2015-09-24T16:15:41Z
dc.date.available 2016-07-16T08:17:17Z
dc.date.issued 2015-07
dc.description Author Posting. © The Author(s), 2015. This is the author's version of the work. It is posted here by permission of Springer for personal use, not for redistribution. The definitive version was published in Extremophiles 19 (2015): 949-960, doi:10.1007/s00792-015-0770-1. en_US
dc.description.abstract The sediment microbiota of the Mediterranean deep-sea anoxic hypersaline basins (DHABs) are understudied relative to communities in the brines and halocline waters. In this study, the active fraction of the prokaryotic community in the halocline sediments of L’ Atalante, Urania, and Discovery DHABs was investigated based on extracted total RNA and 454 pyrosequencing of the 16S rRNA gene. Bacterial and archaeal communities were different in the sediments underlying the halocline waters of the three habitats, reflecting the unique chemical settings of each basin. The relative abundance of unique operational taxonomic units (OTUs) was also different between deep-sea control sediments and sediments underlying DHAB haloclines, suggesting adaptation to the steep DHAB chemical gradients. Only a few OTUs were affiliated to known bacterial halophilic and/or anaerobic groups. Many OTUs, including some of the dominant ones, were related to aerobic taxa. Archaea were detected only in few halocline samples, with lower OTU richness relative to Bacteria, and were dominated by taxa associated with methane cycling. This study suggests that, while metabolically active prokaryotic communities appear to be present in sediments underlying the three DHABs investigated, their diversity and activity are likely to be more reduced in sediments underlying the brines. en_US
dc.description.embargo 2016-07-16 en_US
dc.description.sponsorship This work was supported by NSF OCE- 0849578 to VE and JB and OCE-1061391 to JB and VE. MP was supported by the WHOI postdoctoral scholarship program. KAK was partially supported by the University of Thessaly through a sabbatical in 2013. en_US
dc.format.mimetype application/pdf
dc.identifier.uri https://hdl.handle.net/1912/7545
dc.language.iso en_US en_US
dc.relation.uri https://doi.org/10.1007/s00792-015-0770-1
dc.subject Bacteria en_US
dc.subject Archaea en_US
dc.subject cDNA en_US
dc.subject Activity en_US
dc.subject L’ Atalante en_US
dc.subject Urania en_US
dc.subject Discovery en_US
dc.subject Anoxic en_US
dc.title Inter-­comparison of the potentially active prokaryotic communities in the halocline sediments of Mediterranean deep-sea hypersaline basins en_US
dc.type Preprint en_US
dspace.entity.type Publication
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