Next-generation transgenic mice for optogenetic analysis of neural circuits

Thumbnail Image
Date
2013-11-26
Authors
Asrican, Brent
 Augustine, George J.
 Berglund, Ken
 Chen, Susu
 Chow, Nick
 Deisseroth, Karl
 Feng, Guoping
 Gloss, Bernd
 Hira, Riichiro
 Hoffmann, Carolin
 Kasai, Haruo
 Katarya, Malvika
 Kim, Jinsook
 Kudolo, John
 Lee, Li Ming
 Lo, Shun Qiang
 Mancuso, James
 Matsuzaki, Masanori
 Nakajima, Ryuichi
 Qiu, Li
 Tan, Gregory
 Tang, Yanxia
 Ting, Jonathan T.
 Tsuda, Sachiko
 Wen, Lei
 Zhang, Xuying
 Zhao, Shengli
Linked Authors
Person
Person
Person
Person
Person
Files
fncir-07-00160.pdf (1.94 MB)
Alternative Title
Citable URI
https://hdl.handle.net/1912/6396
As Published
https://doi.org/10.3389/fncir.2013.00160
Date Created
Location
DOI
10.3389/fncir.2013.00160
Related Materials
Replaces
Replaced By
Keywords
Optogenetics
 Channelrhodopsin
 Photostimulation
 Photoinhibition
 Cerebellum
 Cortex
 Hippocampus
 Pons
Abstract
Here we characterize several new lines of transgenic mice useful for optogenetic analysis of brain circuit function. These mice express optogenetic probes, such as enhanced halorhodopsin or several different versions of channelrhodopsins, behind various neuron-specific promoters. These mice permit photoinhibition or photostimulation both in vitro and in vivo. Our results also reveal the important influence of fluorescent tags on optogenetic probe expression and function in transgenic mice.
Description
© The Author(s), 2013. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Frontiers in Neural Circuits 7 (2013): 160, doi:10.3389/fncir.2013.00160.
Embargo Date
Citation
Frontiers in Neural Circuits 7 (2013): 160
Cruises
Cruise ID
Cruise DOI
Vessel Name
Collections
Visiting Investigators
Except where otherwise noted, this item's license is described as Attribution 3.0 Unported