Mitochondrial genotype influences the response to cold stress in the European green crab Carcinus maenas Coyle, Aidan F. Voss, Erin R. Tepolt, Carolyn K. Carlon, David B. 2019-09-05T19:56:36Z 2020-07-08T07:28:40Z 2019-07-08
dc.description Author Posting. © Company of Biologists, 2019. This article is posted here by permission of Company of Biologists for personal use, not for redistribution. The definitive version was published in Journal of Experimental Biology 222(17), (2019):jeb.20352, doi: 10.1242/jeb.203521. en_US
dc.description.abstract Hybrid zones provide natural experiments in recombination within and between genomes that may have strong effects on organismal fitness. On the East Coast of North America, two distinct lineages of the European green crab (Carcinus maenas) have been introduced in the last two centuries. These two lineages with putatively different adaptive properties have hybridized along the coast of the eastern Gulf of Maine, producing new nuclear and mitochondrial combinations that show clinal variation correlated with water temperature. To test the hypothesis that mitochondrial or nuclear genes have effects on thermal tolerance, we first measured the response to cold stress in crabs collected throughout the hybrid zone, then sequenced the mitochondrial CO1 gene and two nuclear single nucleotide polymorphisms (SNPs) representative of nuclear genetic lineage. Mitochondrial haplotype had a strong association with the ability of crabs to right themselves at 4.5°C that was sex specific: haplotypes originally from northern Europe gave male crabs an advantage while there was no haplotype effect on righting in female crabs. By contrast, the two nuclear SNPs that were significant outliers in a comparison between northern and southern C. maenas populations had no effect on righting response at low temperature. These results add C. maenas to the shortlist of ectotherms in which mitochondrial variation has been shown to affect thermal tolerance, and suggest that natural selection is shaping the structure of the hybrid zone across the Gulf of Maine. Our limited genomic sampling does not eliminate the strong possibility that mito-nuclear co-adaptation may play a role in the differences in thermal phenotypes documented here. Linkage between mitochondrial genotype and thermal tolerance suggests a role for local adaptation in promoting the spread of invasive populations of C. maenas around the world. en_US
dc.description.embargo 2020-07-08 en_US
dc.description.sponsorship We would like to thank T. Suskiewicz and L. Johnson for help collecting crabs from Halifax, NS, and Robin Seeley for collections from the Isle of Shoals. We thank Timothy Fuller for designing and testing the SMC primers. We thank Mark Murray for facilitating a productive stay on Kent Island, and Nick Keeney for assistance with animal care at the Schiller Coastal Studies Center. This is publication no. 5 from the Bowdoin Marine Laboratory. en_US
dc.identifier.citation Coyle, A. F., Voss, E. R., Tepolt, C. K., & Carlon, D. B. (2019). Mitochondrial genotype influences the response to cold stress in the European green crab Carcinus maenas. Journal of Experimental Biology, 222(17), jeb.203521. en_US
dc.identifier.doi 10.1242/jeb.203521
dc.publisher Company of Biologists en_US
dc.subject Cold tolerance en_US
dc.subject Gulf of Maine en_US
dc.subject Hybrid zone en_US
dc.subject Mitochondria en_US
dc.subject Mito-nuclear interactions en_US
dc.subject Natural selection en_US
dc.subject OCLTT model en_US
dc.subject Oxygen and capacity-limited thermal tolerance en_US
dc.title Mitochondrial genotype influences the response to cold stress in the European green crab Carcinus maenas en_US
dc.type Article en_US
dspace.entity.type Publication
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